dnase sensitivity assay

1. 2019/12/31 DNase I Footprinting Assay Service - Profacgen  (https://www.profacgen.com) Home (/) Services Products APP & TECH Contact Us About Us (https://www.profacgen.com/contact- (https://www.profacgen.com/about- us.htm) us.htm) DNase I Footprinting Assay Service Home (http://www.profacgen.com) / Services / Protein Analysis (https://www.profacgen.com/protein-analysis.htm) / Nucleic Acid-Protein Interactions  Sign In (https://www.profacgen.com/SignIn.aspx) / Register (https://www.profacgen.com/services/protein-analysis/nucleic-acid-protein- interactions) (https://www.profacgen.com/SignUp.aspx) / DNA-Protein Interactions (https://www.profacgen.com/services/protein- analysis/nucleic-acid-protein-interactions/dna-protein-interactions) / DNase I Footprinting Assay Service https://www.profacgen.com/dnase-i-footprinting-assay-service.htm 1/5

2. 2019/12/31 DNase I Footprinting Assay Service - Profacgen DNase I Footprinting Assay Service Profacgen provides DNase I footprinting assay service for the detection of DNA- protein interaction and identification of the exact binding sites of DNA-binding proteins. DNase I footprinting assay is based on the fact that a DNA-binding protein often protects its bound DNA fragment from enzymatic cleavage, making it possible to locate a protein binding site on a particular DNA fragment. This method uses DNase I to digest the radioactively-labeled or fluorescently- labeled DNA fragment, and then uses gel electrophoresis to detect the resulting cleavage pattern. The cleavage pattern of the DNA in the absence of a DNA binding protein, typically referred to as free DNA, is compared to the cleavage pattern of DNA in the presence of a DNA binding protein. If the protein binds to the DNA fragment, the binding site is protected from enzymatic cleavage. This protection will result in a clear area on the gel which is referred to as a “footprint”. By varying the concentration of the DNA-binding protein, the binding affinity of the protein can be estimated according to the minimum concentration of protein at which a footprint is observed. Schematic Diagram of the DNase I Footprinting Assay https://www.profacgen.com/dnase-i-footprinting-assay-service.htm 2/5

3. 2019/12/31 DNase I Footprinting Assay Service - Profacgen Classic DNase I footprinting assay labels DNA molecules with radioisotope, and need to run sequencing gel for detection. However, as radioactive labeling has safety concern and is not as convenient as fluorescence labeling, Profacgen uses fluorescence labeling (/protein-labeling.htm) in combination with sequencer (e.g. supplied by Applied Biosystem), enabling our DNase I footprinting assay safer, faster and more sensitive. As DNase I footprinting assay is used to determine the precise DNA sequence bound by the protein of interest, it is recommended to be performed after interaction confirmation by Electrophoretic Mobility Shift Assay (EMSA) (/Electrophoretic- Mobility-Shift-Assay-EMSA-Service.htm). Therefore, in addition to purified protein and the DNA fragment, EMSA data and relating experimental parameters will all help design the DNase I footprinting assay. Experiment flowchart: Protein and nucleotide preparation (optional) EMSA verification (optional) https://www.profacgen.com/dnase-i-footprinting-assay-service.htm 3/5

4. 2019/12/31 DNase I Footprinting Assay Service - Profacgen DNase I footprinting Data analysis Result delivery Our Advantages: High sensitivity and reproducible results Highly experienced technicians Tailored experimental design for specific requirements Best price in the market Please feel free to contact us for more information of our professional DNase I footprinting assay service. SERVICES  Protein Production (https://www.profacgen.com/protein-production.htm)   Bioinformatics Platform Introduction  (https://www.profacgen.com/Bioinformatics-Platform-Introduction.htm)  Protein Analysis (https://www.profacgen.com/protein-analysis.htm)  ChIRP-Seq Service (https://www.profacgen.com/services/protein-analysis/chi… * Complement Assays (https://www.profacgen.com/services/protein-analysis/c…  * Enzyme Activity Assay Service (https://www.profacgen.com/enzyme-activity-…  * Nucleic Acid-Protein Interactions (https://www.profacgen.com/services/protei…  * DNA-Protein Interactions (https://www.profacgen.com/services/protein-analysis/…  * Chromatin Immunoprecipitation (ChIP) Assay Service (https://www.profacgen.com/c… DNase I Footprinting Assay Service (https://www.profacgen.com/dnase-i-footprinting… Electrophoretic Mobility Shift Assay (EMSA) Service (https://www.profacgen.com/Ele… https://www.profacgen.com/dnase-i-footprinting-assay-service.htm 4/5

5. 2019/12/31 DNase I Footprinting Assay Service - Profacgen RNA-Protein Interactions (https://www.profacgen.com/services/protein-analysis/…  * Protein Aggregation Analysis (https://www.profacgen.com/protein-aggregati… * Protein Interaction Analysis Services (https://www.profacgen.com/protein-int…  * Screening and Profiling Services (https://www.profacgen.com/screening-and-…  * Transcription Factor Analysis (https://www.profacgen.com/transcription-fact… *  Drug Development (https://www.profacgen.com/drug-development.htm)  INQUIRY (https://www.profacgen.com/order.htm?i1=DNase I Footprinting Assay Service) CONTACT US  Email: info@profacgen.com  Tel: 1-631-448-8149  Fax: 1-631-938-8127  45-1 Ramsey Road, Shirley, NY 11967, USA Copyright ©2010 - 2019 Profacgen. All rights reserved. | Terms and Conditions (https://www.profacgen.com/term-and- conditions.htm) | Privacy Policy (https://www.profacgen.com/privacy-policy.htm) https://www.profacgen.com/dnase-i-footprinting-assay-service.htm 5/5