single-cell-gene-expression-service

1. SINGLECELL RNA (SCRNA) SEQUENCINGSERVICE Single Cell Single Cell Isola�on 1 Recent advances in various single-cell technologies are providing unprecedented opportunities to discover the differences of biological systems at single-cell level. Traditional average data analyzed across bulk populations can not illustrate various crucial biological phenomena. Isolation Technology - Low Flux Isola�on Technology - High Throughput Isolation Workflow b a a Empty well Wel with 1+x cells 10x Barcoded Gel Beads Sheath Flow 96-well MTP Multispectral Detector Tissue Limi�ng Dilu�on Laser Cells b Dissocia�on Charge Laser (focused) Cutting - Deflector Plates - + Single cell RNA (scRNA) sequencing service can be divided into the following three steps: 1) Single Cell Isolation 2) Library Construction and Sequencing 3) Data Analysis Enzyme, Labeled CellsOil Laser Catapulting (defocused) - Cells + LCM c Isola�on Collection Tubes Single Cell GEMs Pipette Cells FACS Micromanipula�on Microfluidic Single Cell Library Construc�on and Sequencing The scRNA-seq enables researchers to characterize transcriptomes at single-cell level, which is especially important for further under- standing the cellular interac- tion networks of tissues and organs. The main advan- tage of scRNA-seq is that high resolution and genome-wide range make it possible to solve problems that are intractable using other methods, such as bulk RNA-seq or single-cell RT-qPCR. 2 RT & Second -strand synthesis Extraction IVT scRNA Sequencing Methods Single Cell RNA cDNA Drop-seq CEL-seq PCR Smart-seq MARS-seq Amplified RNA Tang-seq STRT-seq ????????????????? ????????????????? ????????????????? ????????????????? ????????????????? RT Sequencing Library Construc�on Amplified cDNA Data Analysis 3 GO Gene overlap 5 25 50 75 With unrivaled expertise in precision oncology, Creative Biolabs has developed integrated single cell omics solutions for various fields, facilitating sought-after and innovative approaches to discover the differences between each single cells. Our scientists and techni- cians are dedicated to advancing your single cell-based scientific programs from various omics solutions to big data. Adjusted p-value 10-4 10-6 10-8 10-10 10-12 10-14 Gene Ra�o Cell Subpopula�on Ra�o Analysis Trajectory Analysis of Cell State Gene Expression Profiles Gene Set Analysis Rare Subtype Regulatory Network Analysis Clustering Analysis Immune Response Analysis Rare Subtype Iden�fica�on WHAT CAN WE DO OTHER SERVICES Single Cell Sequencing Service · Characterizing Heterogeneity · Exploring Cancer Immunotherapies · Unravelling Cancer Evolu�on · Monitoring of Immunity · Profiling the Tumor Microenvironment · Virus Entry Mechanism and Therapeu�cs Discovery · Single Cell DNA Sequencing Services · Single Cell Methyla�on Sequencing Services · Single Cell ATAC Sequencing Services · Single Cell Proteomic Analysis singlecell.creative-biolabs.com Email: info@creative-biolabs.com NY， USA