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Preparation of sample libraries for next-generation sequencing!

The primary methods for library preparation include ligation-based library prep, segmentation-based library preparation, and amplicon library preparation. The particular protocol you select depends on your sequencing platform and the downstream analysis. <br>

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Preparation of sample libraries for next-generation sequencing!

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  1. PREPARATION OF SAMPLE LIBRARIES FOR NEXT-GENERATION SEQUENCING! HTTPS://TEKMATIC.COM

  2. Next-generation sequencing allows the profiling of sequences from transcriptomes and genomes and DNA-protein interaction. The techniques used are an integral component of the research process and discoveries in the field of biology. The capability to quickly create large volumes of sequence information offers various applications, speeding advancements in research and transforming our understanding of the human body’s health and diseases.

  3. PREPARING SEQUENCING LIBRARIES FOR SEQUENCING For DNA, or cDNA (synthesized using the RNA), to be sequenced, it must be separated, repaired at the end, and put into libraries for sequencing. Put the term sequencing libraries refers to pools of DNA fragments containing adapter sequences that work with a particular sequencing platform and indexing barcodes that allow for identifying unique samples. The primary methods for library preparation include ligation-based library prep, segmentation-based library preparation, and amplicon library preparation. The particular protocol you select depends on your sequencing platform and the downstream analysis. The most fundamental steps for library preparation include fragmentation, end repair, as well as the addition of adapters, and (optional) Amplification of PCR:

  4. End repair and fragmentation Short-read sequencing techniques like Illumina cannot easily analyze very long DNA strands. As a result, DNA must be broken down into smaller, uniform pieces. After the fragmentation process, the DNA fragments are either repaired or polished at the end. A single Adenine base is added to create an overhang through the A-tailing process. This A overhang permits adapters with only a single thymine base to pair DNA fragments. When performing RNA-seq, RNA must first be transcribed into cDNA. The fragmentation process can be carried out prior to or following cDNA synthesizing.

  5. The addition of adapters occurs after fragmentation (or when it comes to library tagmentation preparation in conjunction with fragmentation). Adapters are permanently attached to the edges of DNA fragments. The adapters have multiple uses. They can transfer sequences into a flow cell, ensure compatibility with specific sequencing platforms, including barcodes (also known as indexes) to help identify the samples, and allow multiplexing in both target enrichment and sequencing. IDT has a range of options available in NGS adapters and indexing primers.

  6. Thank You Address:- 7316 N Alpine Road Loves Park, IL 61111 Phone:- 815.282.1775 Website:- https://tekmatic.com

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