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WT …TGTAGGGCTG… mSA …TGTctGGCTG…

LUC. pA. K30-3'-asLUC. K30-3'-asLUC mSA. A. 900. B. e1. intron. exon 2. M. 750. 3’ LTR. 600. Luciferase activtiy (RLU). WT …TGTAGGGCTG… mSA …TGTctGGCTG…. 450. *. 300. 4. 5. 6. 1. 2. 3. 150. -. -. RT enzyme. +. +. +. +. 0. RT primer. -. +. -. +. -. -.

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WT …TGTAGGGCTG… mSA …TGTctGGCTG…

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  1. LUC pA K30-3'-asLUC K30-3'-asLUC mSA A 900 B e1 intron exon 2 M 750 3’ LTR 600 Luciferase activtiy (RLU) WT …TGTAGGGCTG… mSA …TGTctGGCTG… 450 * 300 4 5 6 1 2 3 150 - - RT enzyme + + + + 0 RT primer - + - + - - K30-3'-asLUC K30-3'-asLUC mSA C WT …TGGATGGCGG… mATG/e1 …TGGAacGCGG… WT …AGCATGGTTA… mATG/int …AGCcTaGTTA… intron exon 2 e1 WT …TAGGTAGGCT… mSD2 …TAGcaAGGCT… WT …CAGGTAGGGC… mSD1 …CAGcaAGGGC… WT …TGTAGGGCTG… mSA …TGTctGGCTG… D SP1 mSA WT HBZ mSD2 mSD1 mATG/e1 mATG/int

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