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Protein Synthesis

Protein Synthesis. One Gene – One Enzyme. Protein Synthesis. Protein Synthesis. Protein Synthesis. Protein Synthesis. Protein Synthesis. Transcription DNA  mRNA Occurs in the nucleus. Translation mRNA  tRNA  AA Occurs at the ribosome. Protein Synthesis. Triplets (Codons).

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Protein Synthesis

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  1. Protein Synthesis

  2. One Gene – One Enzyme

  3. Protein Synthesis

  4. Protein Synthesis

  5. Protein Synthesis

  6. Protein Synthesis

  7. Protein Synthesis

  8. Transcription DNA  mRNA Occurs in the nucleus Translation mRNA  tRNA  AA Occurs at the ribosome Protein Synthesis

  9. Triplets (Codons)

  10. Genetic Code

  11. Transcription

  12. Transcription

  13. Transcription

  14. Transcription

  15. Initiation • Promoter • TATA Box • Initiation Site • Transcription factor • RNA Polymerase • AUG

  16. Elongation • RNA Polymerase • Adds nucleotides to the 3’ end

  17. Termination • Proceeds until RNA polymerase transcribes a terminator sequence • UAA • UAG • UGA

  18. RNA Processing • 5’ guanine cap • 3” Poly (A) tail

  19. RNA Splicing • Introns • Exons

  20. Spliceosomes • snRNPs • Small nuclear ribonucleoproteins

  21. Introns???? • Regulation • One gene can code for alternate polypeptides • Evolution • Domains – structural and functional regions

  22. Translation

  23. tRNA

  24. Aminoacyl-tRNA Synthetase

  25. Ribosomes • Small subunit • Large subunit • P site (peptidyl-tRNA) • A site (Aminoacyl-tRNA) • E site (Exit site)

  26. Initiation

  27. Elongation

  28. Termination

  29. Polyribosomes

  30. Point Mutation

  31. Point Mutation • Base Pair Insertion or Deletion • Frameshift • Missense – new AA • Nonsense – stop

  32. Point Mutation • Base Pair Substitution

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