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Raman Spectroscopy

Raman Spectroscopy. Rahul Joseph Lee Laim. Raman Effect. The incoming light is in-elastically scattered by the vibration of the molecules. This change in frequency of the light due to vibrational scattering is the Raman effect. Mechanism of Scattering. Transitions occur in 10 -14 seconds

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Raman Spectroscopy

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  1. Raman Spectroscopy Rahul Joseph Lee Laim

  2. Raman Effect The incoming light is in-elastically scattered by the vibration of the molecules. This change in frequency of the light due to vibrational scattering is the Raman effect.

  3. Mechanism of Scattering • Transitions occur in 10-14 seconds • Raman Shift (cm-1) • Excess energy released as heat

  4. Raman Microspectroscopy • Sample volume can be as small as 1µm diameter • Imaging modes • Direct Imaging – Imaging over small range of wavenumbers • Hyperspectral Imaging –Location profile of various components

  5. Molecular Fingerprints

  6. Applications • Detect counterfeit drugs • Non-destructive detection of biological components • Monitors gas mixtures in medical applications • Characterization of materials and orientation • Monitors activity in catalytic reactions

  7. 6W Argon Ion Laser 457.9 nm, 465.8 nm,, 488.0 nm, 496.5 nm, 514.5 nm, 528.7 nm.

  8. Laser Light Path

  9. Photomultiplier Tube

  10. Spectrometer (monochrometer) nλ=2dsinΘ

  11. Dye Laser

  12. Questions

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