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Caffeine Content Case Study — A case study on the caffeine content in brewed tea

Caffeine is a xanthine derivative with a chemical name of 1,3,7-trimethylxanthine, an alkaloid extracted from tea or coffee. It can excite the cerebral cortex in the brain for temporary excitement to drive away drowsiness and restore energy. Learn more at https://www.alfachemic.com/testinglab/_resource/flyer/caffeine-content-case-study-com.html

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Caffeine Content Case Study — A case study on the caffeine content in brewed tea

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  1. Caffeine Content Case Study — A case study on the caffeine content in brewed tea Background Method Caffeine is a xanthine derivative with a chemical name of 1,3,7- We analyzed the samples using high-performance liquid trimethylxanthine, an alkaloid extracted from tea or coffee. It can chromatography (HPLC) according to the ISO 20481 standard. excite the cerebral cortex in the brain for temporary excitement Caffeine was extracted from samples with water at 90 °C in the to drive away drowsiness and restore energy. Caffeine- presence of magnesium oxide. After filtration, the caffeine containing beverages such as coffee, tea, and soft drinks are content of the extract was determined by HPLC on an RP-18 prevalent. Food Standards Australia and New Zealand (FSANZ) column using isocratic elution with UV detection at regulations posted a prohibition of selling the retail sale of food approximately 272 nm. with a total caffeine content of 5% or more (solid or semi-solid Report of Analysis food) or 1% or more (liquid food), effective December 12, 2019. Sample No. Test Item Result Reporting Limit Method The caffeine-added cola-type beverages and caffeinated energy drinks are still allowed if the maximum amount complies with the Brewed Tea A Caffeine 5.17 mg/oz 1.50 mg/oz ISO 20481 regulations. Brewed Tea B Caffeine 6.65 mg/oz 1.50 mg/oz ISO 20481 Brewed Tea C Caffeine 3.40 mg/oz 1.50 mg/oz ISO 20481 Table 1. Results from the analysis of caffeine content in three tea samples Summary The client provided the brewed tea samples for a contracted analysis of caffeine content. We analyzed and tested three groups of samples. Results are shown in Table 1. Contact Us Room 1, 2200 Smithtown Avenue, Ronkonkoma, NY 11779-7329, USA Case Email: info@alfa-chemistry.com Presented here is a case study in which we analyzed in our Web: https://www.alfachemic.com/testinglab/industries/ laboratory the caffeine content in brewed tea for a client. coffee-analysis.html

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