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JAXA Protein Crystal Growth Experiment (JAXA PCG)

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JAXA Protein Crystal Growth Experiment (JAXA PCG)

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  1. Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA). JEM PCG 31407 JAXA Protein Crystal Growth Experiment(JAXA PCG) Instructor: Takashi Yamasaki Yuki Kawai Takuya Takami (yamasaki.takashi@jaxa.jp)(yuki.kawai@jamss.co.jp) (takami.takuya@jaxa.jp)

  2. Objectives After this lesson the trainee will be able to: • JAXA PCG Experiment • Canister • JAXA PCG Operations Overview • Key points during JAXA PCG Operations • JAXA PCG Operations • Explain the science objective of JAXA PCG experiment. • List onboard outcome from performing JAXA PCG experiment. • Explain the function of Canister. • List crew tasks regarding JAXA PCG experiment. • Explain what crew must pay attention to while handling Canister. • Explain what crew must pay attention to while inserting Canister into Canister Bag. • Perform JAXA PCG experiment tasks according to ODF.

  3. Lesson Outline Contents: - JAXA PCG Experiment - Canister - JAXA PCG Operations Overview - Key points during JAXA PCG Operations - Acronyms - Appendix Page: 4 5 6 7 8 9

  4. Crystallized in ISS Crystallized on Ground Crystals of alpha-amylase and electron density map (under studying) Protein Crystallization Research Facility (PCRF) in Ryutai Rack JAXA PCG Experiment The main objective of JAXA PCG Experiment - To get the high quality protein crystals under microgravity to bring more precise protein 3 dimensional structure. Onboard outcomes from JAXA PCG experiment - Protein crystals grown in JEM Protein Crystallization Research Facility (PCRF).

  5. Canister Canister - Canister is one of PCRF Crystal Growth Cell dedicated to JAXA PCG Experiment. - Function of the Canister is to: - control temperature to keep crystallization condition.(20 degrees Celsius plus or minus 2 degrees) - Counter diffusion method is used for protein crystallization. - Many kinds of protein solutions are in a Canister. or (TBD) Canister Canisters installed in PCRF Cell Tray

  6. JAXA PCG Operations Overview Russian Crewmember Operations NASA/IP Crewmember Operations Ground Operations Prepare Canister(Start Crystallization Process)and launch it by Progress Canister Retrieval from Progress - Retrieve Canister from Progress.- Hand over Canister to NASA/IP Crewmember. Canister Installation - Install Canister into PCRF. PCRF Activation Temperature control (Experiment) PCRF Deactivation Canister Removal - Remove Canister from PCRF.- Hand over Canister to Russian Crewmember. Canister Return by Soyuz - Install Canister into Soyuz.- Return Canister to ground.

  7. Key points during JAXA PCG Operations

  8. IPODFPCGPCRF International PartnerOperations Data FileProtein Crystal GrowthProtein Crystallization Research Facility Acronyms

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