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Chapter 14

Chapter 14. Mechanisms of Enzyme Action Biochemistry by Reginald Garrett and Charles Grisham. Essential Question. Although the catalytic properties of enzymes may seem almost magical, it is simply chemistry– the breaking and making of bonds – that give enzymes their prowess

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Chapter 14

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  1. Chapter 14 Mechanisms of Enzyme Action Biochemistry by Reginald Garrett and Charles Grisham

  2. Essential Question Although the catalytic properties of enzymes may seem almost magical, it is simply chemistry– the breaking and making of bonds– that give enzymes their prowess • What are the universal chemical principles that influence the mechanisms of enzymes • To understand the enormous catalytic power of enzymes

  3. Outline of Chapter 14 • What Are the Magnitudes of Enzyme-Induced Rate Accelerations? • What Role Does Transition-State Stabilization Play in Enzyme Catalysis? • How does Destabilization of ES Affect Enzyme Catalysis? • How Tightly Do Transition-State Analogs Bind to the Active Site? • What Are the Mechanisms of Catalysis? • What Can Be Learned from Typical Enzyme Mechanisms?

  4. 14.1 – What Are the Magnitudes of Enzyme-Induced Rated Accelerations?

  5. Enzymes are powerful catalysts • The large rate accelerations of enzymes (107 to 1015) correspond to large changes in the free energyof activation for the reaction • All reactions pass through a transition state on the reaction pathway • The active sites of enzymes bind the transition state of the reaction more tightly than the substrate, by doing so, enzymes stabilize the transition state and lower the activation energy of the reaction H-O-H + Cl- H-O H Cl HO- + HCl Reactant Transition state Products d- d-

  6. 14.2 – What Role Does Transition-State Stabilization Play in Enzyme Catalysis? Figure 14.1Enzymes catalyze reactions by lowering the activation energy. Here the free energy of activation for (a) the uncatalyzed reaction, DGu‡, is larger than that for (b) the enzyme-catalyzed reaction, DGe‡.

  7. The catalytic role of an enzyme is to reduce the energy barrier between substrate S and transition state • Rate acceleration by an enzyme means that the energy barrier between ES and EX‡ must be smaller than the barrier between S and X‡ • This means that the enzyme must stabilize the EX‡ transition state more than it stabilizes ES • Enzymes bind the transition state structure more tightly than the substrate

  8. 14.3 – How does Destabilization of ES Affect Enzyme Catalysis? • The favorable interactions between the substrate and amino acid residues on the enzyme account for the intrinsic binding energy, DGb • The intrinsic binding energy ensures the favorable formation of the ES complex • If uncompensated, it makes the activation energy for the enzyme-catalyzed reaction unnecessarily large and wastes some of the catalytic power of the enzyme

  9. intrinsic binding energy Figure 14.2The intrinsic binding energy of the enzyme-substrate (ES) complex (DGb ) is compensated to some extent by entropy loss due to the binding of E and S (TDS) and by destabilization of ES (DGd) by strain, distortion, desolvation , and similar effects. If DGb were not compensated by TDS and DGd, the formation of ES would follow the dashed line.

  10. Figure 14.3 (a) Catalysis does not occur if the ES complex and the transition state for the reaction are stabilized to equal extents. (b) Catalysis will occur if the transition state is stabilized to a greater extent than the ES complex (right). Entropy loss and destabilization of the ES complex DGd ensure that this will be the case.

  11. What Roles Do Entropy Loss and Destabilization of the ES Complex Play? Raising the energy of ES raises the rate • For a given energy of EX‡, raising the energy of ES will increase the catalyzed rate • This is accomplished in two ways: • loss of entropy due to formation of ES • destabilization of ES by • structural strain & distortion • desolvation • electrostatic effects

  12. Figure 14.4(a) Formation of the ES complex results in a loss of entropy. Prior to binding, E and S are free to undergo translational and rotational motion. By comparison, the ES complex is a more highly ordered, low-entropy complex. -(TDS)

  13. Figure 14.4(b) Substrates typically lose waters of hydration in the formation of the ES complex. Desolvation raises the energy of the ES complex, making it more reactive. Figure 14.4(c) Electrostatic destabilization of a substrate may arise from juxtaposition of like charges in the active site. If such charge repulsion is relieved in the course of the reaction, electrostatic destabilization can result in a rate increase.

  14. 14.4 – How Tightly DoTransition-State Analogs Bind to the Active Site? • Transition state is exists only for about 10 -13sec, less than the time required for a bond vibration • The nature of the elusive transition state can be explored using transition state analogs • Transition state analogs are stable molecules, chemically and structurally similar to the transition state • Transition-state analogs are only approximations of the transition state itself and will never bind as tightly as would be expected for the true transition state

  15. Transition-State Analogs Figure 14.5The proline racemase reaction. Pyrrole-2-carboxylate and D -1-pyrroline-2-carboxylate mimic the planar transition state of the reaction.

  16. Figure 14.6(a) Phosphoglycolohydroxamate is an analog of the enediolate transition state of the yeast aldolase reaction. (b) Purine riboside, a potent inhibitor of the calf intestinal adenosine deaminase reaction, binds to adenosine deaminase as the 1,6-hydrate. The hydrated form of purine riboside is an analog of the proposed transition state for the reaction.

  17. Transition-State Analogs Make Our World Better • Enzymes are often targets for drugs and other beneficial agents • Transition state analogs often make ideal enzyme inhibitors (p424-425) • Enalapril and Aliskiren lower blood pressure • Statins (Lipitor) lower serum cholesterol • Protease inhibitors are AIDS drugs • Juvenile hormone esterase is a pesticide target • Tamiflu is a viral neuraminidase inhibitor

  18. 14.5 – What Are the Mechanisms of Catalysis? • Enzymes facilitate formation of near-attack conformations • Covalent catalysis • General acid-base catalysis • Metal ion catalysis • Low-barrier hydrogen bonds

  19. 1. Enzymes facilitate formation of near-attack conformations • The enzyme active-site structure and dynamics have emerged from X-ray crystal structures and computer simulations of molecular conformation and motion at the active site • Near-attack conformations (NACs) • Thepreorganization of active site allow it to select and stabilize substrate conformations in which the reacting atoms are in van der Waals contact and at an angle resembling the bond to be formed in the transition state • NACs are precursors to transition states of reactions • Potential reactant molecules adapt a NAC only 0.0001% (without enzyme), NACs form in enzyme active sites from 1% to 70%

  20. Figure 14.7 NACs are characterized as having reacting atoms within 3.2 Å and an approach angle of ±15° of the bonding angle in the transition state.

  21. 1.8Å Figure 14.8 The active site of liver alcohol dehydrogenase (ADH) – a near-attack complex. NAD+ + CH3CH2OH NADH + H+ + CH3CHO

  22. Protein motions are essential to enzyme catalysis • Proteins are constantly moving (p165; Table6.2)– bonds vibrate, side chains bend and rotate, backbone loops wiggle and sway, and whole domains move as a unit • Enzymes depend on such motions to provoke and direct catalytic events • Protein motions support catalysis in several ways: Active site conformation changes can • Assist substrate binding • Bring catalytic groups into position • Induce formation of NACs • Assist in bond making and bond breaking • Facilitate conversion of substrate to product

  23. 2. Covalent catalysis • Some enzyme reactions derive much of their rate acceleration from the formation of covalent bonds between enzyme and substrate BX + Y  BY + X BX + Enz  E:B + X + Y  Enz + BY • Most enzymes that carry out covalent catalysis have ping-pong kinetic mechanisms

  24. The side chains of amino acids in proteins offer a variety of nucleophilic centers for catalysis, including amines, carboxylate, aryl and alkyl hydroxyls, imidazoles, and thiol groups • These groups are readily attack electrophilic centers of substrates, forming covalently bonded enzyme-substrate intermediate

  25. Figure 14.11 Examples of covalent bond formation between enzyme and substrate. In each case, a nucleophilic center (X:) on an enzyme attacks an electrophilic center on a substrate.

  26. 3. General acid-base catalysis • Specific acid-base catalysis involves H+ or OH- that diffuses into the catalytic center • General acid-base catalysis involves acids and bases other than H+ and OH- • These other acids and bases facilitate transfer of H+ in the transition state • Histidine is often the most effective general acid or base

  27. Figure 14.12 Catalysis of p-nitrophenylacetate hydrolysis can occur either by specific acid hydrolysis or by general base catalysis.

  28. 4. Metal ion catalysis • Many enzymes require metal ions for maximal activity (metalloenzymes) • Stabilizing the increased electron density or negative charge • Provide a powerful nucleophile at neutral pH M2++ NucH M2+(NucH) M2+(NucH) + H+

  29. Figure 14.14 Thermolysin is an endoprotease with a catalytic Zn2+ ion in the active site. The Zn2+ ion stabilizes the buildup of negative charge on the peptide carbonyl oxygen, as a glutamate residue deprotonates water, promoting hydroxide attack on the carbonyl carbon.

  30. 5. Low-Barrier Hydrogen Bonds • The typical H-bond strength is 10-30 kJ/mol, and the O-O separation is typically 0.28 nm • As distance between heteroatoms becomes smaller (<0.25 nm), H bonds become stronger • Stabilization energies of LBHB may approach 60 kJ/mol in solution • pKa values of the two electronegative atoms must be similar • Energy released in forming an LBHB can assist catalysis

  31. Low-barrier hydrogen bond (LBHB) • The typical strength of a hydrogen bond is 10 to 30 kJ/mol 0.25 nm (LBHB) 0.5 order 0.1 nm 0.18nm 1 order 0.07

  32. 14.6 – What Can Be Learned from Typical Enzyme Mechanisms? • Serine proteases and aspartic proteases are good examples • Knowledge of the tertiary structure of an enzyme is important • Enzymes are the catalytic machines that sustain life

  33. The Serine Proteases Trypsin, chymotrypsin, elastase, thrombin, subtilisin, plasmin, TPA • Serine proteases are a class of proteolytic enzymes whose catalytic mechanism is based on an active-site serine residue • Ser is part of a "catalytic triad" of Ser, His, Asp • Serine proteases are homologous, but locations of the three crucial residues differ somewhat • Enzymologists agree, however, to number them always as His-57, Asp-102, Ser-195

  34. Figure 14.17The catalytic triad of chymotrypsin . Figure 14.16 Structure of chymotrypsin (white) in a complex with eglin C (blue ribbon structure), a target protein. The residues of the catalytic triad (His57, Asp102, and Ser195) are highlighted. His57 (blue) is flanked above by Asp102 (red) and on the right by Ser195 (yellow). The catalytic site is filled by a peptide segment of eglin. Note how close Ser195 is to the peptide that would be cleaved in a chymotrypsin reaction.

  35. Figure 14.18 The substrate-binding pockets of trypsin, chymotrypsin, and elastase.

  36. Serine Protease Mechanism A mixture of covalent and general acid-base catalysis • Asp-102 functions only to orient His-57 • His-57 acts as a general acid and base • Ser-195 forms a covalent bond with peptide to be cleaved • Covalent bond formation turns a trigonal C into a tetrahedral C • The tetrahedral oxyanion intermediate is stabilized by N-Hs of Gly-193 and Ser-195

  37. Serine Protease Mechanism Kinetics • The mechanism is based on studies of the hydrolysis of artificial substrates– simple organic ester • In the chymotrypsin mechanism, the nitrophenylacetate combines with the enzyme to form an ES complex • Followed by a rapid second step in which an acyl-enzyme intermediate is formed, with the acetyl group covalently bonded to the very reactive Ser-195

  38. Serine Proteases Display Burst Kinetics Multistep mechanism Figure 14.20 Burst kinetics in the chymotrypsin reaction.

  39. Figure 14.23A detailed mechanism for the chymotrypsin reaction. Note the low-barrier hydrogen bond (LBHB) in (c) and (g). ES complex Tetrahedral oxyanion transition state Acyl-enzyme intermediate Acyl-enzyme-H2O intermediate Tetrahedral oxyanion transition state

  40. The Serine Protease Mechanism in Detail Binding of substrate.

  41. The Serine Protease Mechanism in Detail Trigonal C The formation of the covalent ES complex involves general base catalysis by His57

  42. The Serine Protease Mechanism in Detail Tetrahedral C His57 stabilized by a LBHB. The “oxyanion hole”: amide hyrdogens of Ser195 & Gly193

  43. The Serine Protease Mechanism in Detail Collapse of the tetrahedral intermediate releases the first product.

  44. The Serine Protease Mechanism in Detail The amino product departs, making room for an entering water molecule.

  45. The Serine Protease Mechanism in Detail Nucleophilic attack by water is facilitated by His57, acting as a general base.

  46. The Serine Protease Mechanism in Detail Collapse of the tetrahedral intermediate cleaves the covalent intermediate, releasing the second product.

  47. The Serine Protease Mechanism in Detail Carboxyl product release completes the serine protease mechanism.

  48. The Serine Protease Mechanism in Detail At the completion of the reaction, the side chains of the catalytic triad are restored to their original states.

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