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Hiding in Plain Site: The Immune System, Tuberculosis and Antibiotic Resistance Mark Stephansky

Hiding in Plain Site: The Immune System, Tuberculosis and Antibiotic Resistance Mark Stephansky Whitman-Hanson Regional High School Whitman, Massachusetts. Overall Program Objective.

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Hiding in Plain Site: The Immune System, Tuberculosis and Antibiotic Resistance Mark Stephansky

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  1. Hiding in Plain Site: The Immune System, Tuberculosis and Antibiotic Resistance Mark Stephansky Whitman-Hanson Regional High School Whitman, Massachusetts

  2. Overall Program Objective • To develop and test an immunology-based educational unit designed for first and/or second year high school biology students based on my AAI summer research experience

  3. Curriculum Overview • Cruikshank Lab: T cell migration and function during tuberculosis infection • A third of the world’s population is infected with the TB bacterium; one in 10 of them will become sick with active TB in their lifetime. • TB is responsible for a death every 20 seconds. • An estimated 490,000 new cases of TB resistant to first-line drugs and 40,000 cases of TB resistant to second-line drugs occur every year. (SciAm – March 2009)

  4. Curriculum Overview - continued • TB Infection - entry point for Immune System Study • 21st Century Skills: • MCAS, AP, NEASC • Lab Components: • ELISA - importance as common lab assay and in diagnosing disease • Bacterial transformation

  5. Curriculum Time Requirement: • Total time approximately 7 class periods (computer exercises may be completed by students at home or at the library). • Opening Lecture - 1 class period • Immune system web tutorial - 1 class period • Antibody web investigation - 1 class period • Virtual ELISA - 1 class period • ELISA Lab - 1 class period • Bacterial transformation lab requires 2 lab periods.

  6. Curriculum Overview 1 • Immune System Basics • Innate immunity • Acquired Immunity • TB infection • Harvard Outreach video • "Understanding Resistance to Tuberculosis" • Dr. Barry Bloom • http://outreach.mcb.harvard.edu/videos.htm

  7. Curriculum Overview 2 • Immune system web tutorial • Students will be able to compare and contrast the cell components as well as organs of the immune system.

  8. Curriculum Overview 3 • Antibody web investigation • Students will be able to distinguish between antigens and antibodies. • Lead in for ELISA

  9. Curriculum Overview 4 • ELISA • Virtual HHMI Immunology Lab • Simulated HIV/AIDS • Students will be able to describe the basics of an ELISA assay and practical uses of this type of lab assay.

  10. Curriculum Overview 5 • Bacterial transformation lab • Component of AP Biology • Link to antibiotic resistance • Evolution The LB + pAMP plate shows lawn growth on the lower left. The LB - pAMP plate shows lawn growth on the lower right. The Experimental plate, LB/AMP + pAMP plate shows colonies on the upper left. The LB/AMP - pAMP plate shows no growth on the upper right.

  11. Assessment • Narrative Essays • You are to write a 3-4 page story in the first person (call the protagonist “I”) describing yourself as one who contracts tuberculosis, suffers from the disease and then begins a regimen of antibiotics to cure the disease. • Now, write the same story (3-4 pages) from the point of view of the germ! What do you as the causer of the disease hope to accomplish? What is your experience inside the human body? How did you get there? Was it easy or difficult? Did you mean to do it? What are your worries and concerns regarding the immune system? (Did a macrophage eat any of your friends?)

  12. Assessment – Sample I

  13. Assessment – Sample II

  14. Strengths of Curriculum Unit: • Multi-part Introductory unit • Use or Delete • 21st Century Skills • Web-Based activities • Hands-on Activities • Provides many tie-ins to other curriculum units • Wellness and Disease • Protein Synthesis • Antibiotic Resistance • Evolution

  15. The Future: • Fine Tune Unit: • 21st Century Skills • Google Docs • Broaden Disease Category • Not just TB • Students pick – increase buy in/interest • Expand Antibody/Antigen connection • Protein Shape • Better ELISA Lab activity • AAI – Ann Brokaw?

  16. The AAI Experience: • Fantastic program! • Real Lab Access • Engaging Anecdotes • A new look at Immunology Curriculum Objectives and material • Impact on biology courses at all levels: AP Biology, Academic Biology, A&P

  17. Acknowledgements: • Dr. William Cruikshank, Pulmonary Center, Boston University School of Medicine • Jillian Richmond, BUSM • Cruikshank Lab Team • Matthew Murphy, BU HS Summer Intern • Whitman-Hanson Regional HS • American Association of Immunologists and the John H. Wallace High School Teachers Summer Research Program

  18. ANY QUESTIONS?

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