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Treating Peptone from Heparin Extraction September 14, 2010.
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HeparinFrom WikipediaHeparin, a highly-sulfated glycosaminoglycan, is widely used as an injectable anticoagulant, and has the highest negative charge density of any known biological molecule. It can also be used to form an inner anticoagulant surface on various experimental and medical devices such as test tubes and renal dialysis machines. Pharmaceutical grade heparin is derived from mucosal tissues of slaughtered meat animals such as porcine (pig) intestine or bovine (cow) lung.
MucosaFrom Webster’sMucosa, of mucousMucousFrom Webster’sMucous, 1. covered with or as with mucus. 2. of, or relating to, or resembling mucus. 3. secreting or containing mucus.
PeptoneFrom Webster’sPeptone, any of various water-soluble products of partial hydrolysis of proteins. PeptoneFrom Mike’sPeptone, Waste remaining after Heparin extraction from Mucosa. High in organic loading, high in moisture content, not easily treated or disposed of.
Raw Material:The average weight of the entire small intestine bundle is 5.5 pounds. The separated small intestine, referred to as a runner, from the bundle weighs an average of 4 pounds. The average amount of intestinal mucosa harvested from each runner weighs 2 pounds. The outer covering of the runner, referred to as casing skin, individually weighs an average of 0.5 pound. Dependent on the moisture content of the casing skins upon collection, typically there is an additional 0.5 pound of water present with the skins. This accumulates to a total of 3 pounds total raw material collected from each runner off the casing line.1 gallon of raw material weighs 8.75 pounds.
Notice the white material on the outside of the casings, at our facility some refer to them as strings.
Skins Grinder: Kolbe D5 grinder used to produce finely ground casing skins that can be preserved and digested properly.
Highlights of the digestion process: Collected raw material must be held at 130°F. to stay properly preserved prior to digestion.Collected raw material must be mixed with sufficient sodium bisulfite solution to stay properly preserved prior to digestion. This should be done as soon as possible after collecting from the casing machines. NOTE: 1 % preservation is only sufficient when the raw material is to be digested within 8 hours of start of collection. If raw material is to be held for longer, it should be preserved with 2% bisulfite.
Wastewater Treatment Plant For the Milan facility the apparent best approach was to run a dedicated line to our Anaerobic System. In doing so we will get the least amount of biosolids production, the maximum amount of biogas production, and the least amount of oxygen demand at our Aeration Basins.
Wastewater Treatment Plant From the perspective of flow, the peptone has little effect; however, from the perspective of CBOD and TKN, it is about the equivalent to slaughtering 22% more hogs or around 2,200 head.
Notice the two larger clarifiers in the foreground; they show a light layer of floating foamy scum. This was an unusual development.
From the previous slide this is the clarifier on the left. This picture was taken on January 15, 2010
From the previous slide this is the clarifier on the right. This picture was also taken on January 15, 2010
Best, although not always an available option for dealing with Peptone: Market it and send it off site, which we were able to do starting January 12th. Unfortunately that option ended on June 7th, challenging us with on-site treatment yet again.
Additional Possibilities: RenderingSide stream just the Peptone flow and attempt to float on small DAFLand ApplicationMix the Peptone with our Waste Activated Sludge, then dewater on Belt Filter Press, sending sludge cake to RenderingContinue in an effort to locate some beneficial use/customer
Rendering PossibilitiesAssuming the peptone is 86.2% moisture, it would require around:10,000 hogs/day X 3.13 lb/hog X .0862 (moisture) X 0.92 (dryness) X 1.5 lb 100 psi steam/lb water = 37,233 lb/100 psi steam/day X $11/1000 lb steam = $410/dayValue of dried protein from Peptone = 10,000 hogs/day X 3.13 lb/hog X (1-0.862) X 0.61 (% protein)= 2,635 lb protein/day X $0.20/lb protein=$527/day
Rendering PossibilitiesUnfortunately for the Milan facility in June 2009 the Rendering building is not able to tackle the additional loading of 37,233 pounds of steam required per day.However through operational improvements in June of 2010 when challenged with the same task they have been able to come through for us.
Looking for an opportunity to lessen the amount of moisture sent to our Rendering facility we continue to research methods to separate / concentrate the organic loading.
Coagulated Peptone poured onto screen for gravity separation
Unfortunately when pressure is applied to coagulated Peptone to simulate the action of a dewatering press, the material blinds over the screen a significant amount.
Our next trial was to blend the Peptone with Waste Activated Sludge.
The combined material dewaters extremely well in the laboratory trials.
Within the next few weeks we plan for a full scale test across our filter press.
Processes utilized by a few other facilities One facility utilizes a gravity separator to precipitate the solids for later landfill, while the supernatant flows the POTW. A facility who is currently processing the Peptone through their direct discharge treatment plant, yet struggled through last winter much in the same fashion as Milan, has located a POTW who would process the material for them. The cost estimates for this service is $1350 - $1500 per day depending on transportation fees. There are some facilities who have the treatment capacity to process the Peptone without incident.
