8 mM ATP 0 mM ATP

1. Supplemental Fig 2. Wu et al B A P=0.1817 NS (n=3) SI-induced current (pA/pF) at -100 mV SI-induced current (pA/pF) at -100 mV C P=0.11 NS (n=5) ■ □ 8 mM ATP 0 mM ATP • Supplemental Figure 2.The SI-stimulated channel is pH- and ATP-independent • Pollen protoplasts were tested for SI-induced currents in response to incompatible PrsS, first in standard Ca2+-containing saline (pH 5.5) and then in saline in which pH had been adjusted to 4.5. Each bar shows mean PrsS-induced current at -100 mV (mean ± s.e.m., n=5). • Pollen protoplasts responses to incompatible PrsS were tested first in standard Ca2+-containing saline, (pH 5.5) and then in saline in which pH had been adjusted to 7.5 Each bar shows the PrsS-induced current at -100 mV (mean ± s.e.m., n=3). • The mean I-V relationship of SI-induced current obtained from protoplasts bathed in standard Ca2+-containing saline and dialysed with ATP-free pipette saline (open squares (□), mean ± s.e.m., n=3). Filled squares (■) show the response protoplasts dialysed with standard pipette saline (8 mM ATP, n=6).