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Microscope Math

Microscope Math. Biology 11. Don’t forget to always use units with numbers!. ‘times larger’ or ‘x’ mm µm. Total Magnification Review. Formula: Total Magnification = (eyepiece)(objective) Low: (10)(4) = 40x Medium (10)(10) = 100x High (10)(40) = 400x. Field of View.

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Microscope Math

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  1. Microscope Math Biology 11

  2. Don’t forget to always use units with numbers! ‘times larger’ or ‘x’ mm µm

  3. Total MagnificationReview Formula: Total Magnification = (eyepiece)(objective) Low: (10)(4) = 40x Medium (10)(10) = 100x High (10)(40) = 400x

  4. Field of View • Diameter (widest distance across) of the circle of light when looking through the microscope • Expressed in µm (micrometers)

  5. Micrometers (µm) • 1 µm = 0.001 mm • 1 mm = 1000 µm • To convert: Move decimal 3 places! • Example: 7752 µm = 7.752mm

  6. Measuring the Field Diameter (Low Power) • Place ruler across center of field of view • Line up the middle of a mm mark against one side of field of view • Count the number of full mm spaces. • Estimate amount of next mm if not complete • Record results on board

  7. Field Diameter We will use the following field diameter sizes. Record the µm measurements on your sheet.

  8. Estimating Specimen Size • If you know how wide your field of view is you can estimate the size of specimens being viewed • Expressed in µm (micrometers)

  9. Estimating Specimen Size Formula: Estimated length = Field Diameter (µm) Fit number

  10. What is a “fit number”? • NUMBER of times an object FITS across the field of view • No units are used!

  11. Example • How many times can this ball fit across the field of view? Fit number is 3

  12. If we viewed the ball with low power … Formula: Estimated length = Field of View (µm) Fit number = 4200 µm 3 = 1400 µm Because it was viewed on low power

  13. Any Questions? • Let’s practice!

  14. Diagram Magnification • How many times larger your diagram (or scientific drawing) is than the actual specimen • Expressed as “times larger” or “X”

  15. Diagram Magnification Formula: Diagram magnification = Diagram size (µm) Estimated Size

  16. What is the “diagram size”? • How large your diagram (or scientific drawing) • Expressed in µm • How to find? • Measure your diagram in mm • Multiply by 1000

  17. Example • Diagram the “specimen” below. • Determine the fit number. • Calculate the estimated size.

  18. Example (cont.) • Determine the diagram size in micrometers. • Calculate the diagram magnification.

  19. Any Questions? • Let’s practice!

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