Gene Expression

1. Gene Expression CHS superfamily genes from the moss P. patens Gene Gene location Seq. identity Remarks Scaffold:base to PpCHS (%) PpCHS1a 500:7515799.2 No intron. Differ at 3 a.a. from PpCHS PpCHS01 22:1226899.5 Differ at 2 a.a. and 3′-UTR from PpCHS1a PpCHS1c 426:23863799.2 Differ at 3′-UTR from PpCHS1a PpCHS2a 463:9983894.0 One intron splits Cys72 PpCHS2b.1 463:12962494.0 Differ at 5′-UTR from PpCHS2a PpCHS2c 1:8118194.0 Differ at 3′-UTR from PpCHS2a PpCHS3.1 303:6937889.6 No intron PpCHS3.2 303:7931989.6 Another copy of PpCHS3.1 PpCHS3.3 292:105089.6 Another copy of PpCHS3.1 PpCHS4 39:1774363 91.6 No intron PpCHS5.1 365:13178390.1 No intron PpCHS5.2 365:15177790.1 Another copy of PpCHS5.1 PpCHS6 228:27634985.5 One intron splits Cys76 PpCHS7 425:8287489.7 No intron PpCHS9 25:249014142.1 One intron splits Cys77 PpCHS10 304:41981238.2 Two introns split Cys88 and Gly333 PpCHS11 34:106656733.0 Two introns split Cys80 and Arg348 PpCHS 410:292865 100 49 bp 5′-UTR, 847 bp cds PpCHS13b 16:2859768 (100) 506 bp 5′-UTR, 369 bp cds PpCHS13c 16:2867184 1142 bp containing 228 bp 3′-UTR PpCHS2b.2 76:1642269 492 bp cds, another copy of PpCHS2b.1

2. Light Dark Rebound Light Responsiveness of PpCHS genes PpCHS genes