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Making sense of a gene fusion event in Proteobacteria

Making sense of a gene fusion event in Proteobacteria. Dawn Anderson (Berea College) Rob Barber (U of Wisconsin Parkside) Teresa Johnson (College of Wooster). A promiscuous protein domain (and coding region). The GAT-1 family is comprised of proteins containing a Type-1 glutamine

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Making sense of a gene fusion event in Proteobacteria

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  1. Making sense of a gene fusion event in Proteobacteria Dawn Anderson (Berea College) Rob Barber (U of Wisconsin Parkside) Teresa Johnson (College of Wooster)

  2. A promiscuous protein domain (and coding region) The GAT-1 family is comprised of proteins containing a Type-1 glutamine amidotransferase-like domain. Proteins in this family display an alpha/beta hydrolase fold with a nucleophilic elbow. This transferase-like domain can be present alone or as a fusion with several other protein domains. A fusion between this domain and an AraC DNA binding domain has been found in prokaryotes.

  3. Domain fusion to ArgF DNA binding domain restricted to Proteobacteria

  4. Phylogeny suggests divergence prior to speciation

  5. N-terminal domain phylogeny

  6. Gene associations/neighborhoods offer little functional inference

  7. Approaches to test gene product function • Clone gene, express and purify predicted gene product (trying heparin chromatography) • Structure determination and model building exercises • Gene expression experiments possible (microarrays, proteomics) but likely offer little insight due to prediction of regulatory role

  8. FusionDB offered little insight regarding this protein family

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