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GAP-repair HR

A. Sal I. Sph I. Hin dIII. pBlueIIKS+-GAP repair rescue construct. or i. amp r. F24o1. STM. PPR. 564bp. 479bp. C. Restriction digestion analysis of independent GAP repair recombinations into the pBlueIIKS+ construct. GAP-repair HR. B. M2. M1. 1. 2. 3. M1. 1. 2. M1. 3. kb.

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GAP-repair HR

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  1. A SalI SphI HindIII pBlueIIKS+-GAP repair rescue construct ori ampr F24o1 STM PPR 564bp 479bp C Restriction digestion analysis of independent GAP repair recombinations into the pBlueIIKS+ construct. GAP-repair HR B M2 M1 1 2 3 M1 1 2 M1 3 kb 48.5 kb 38.4 29.9 10.0 24.6 6.0 19.4 15.0 3.0 10.0 2.0 6.0 3.0 EcoRV digests. Standard gel results PmeI digests. FIGE results Lane 3 shows the restriction patterns as expected from the correct recombination event in pBlueAtSTM17 as in B

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