1 / 19

семинары “molecular biology of the cell”

семинары “molecular biology of the cell”. Федор Северин , корпус А, комн. 220 тел. 939-31-07 8-915-120-7955 E-mail: severin@belozersky.msu.ru Святослав Соколов, Дмитрий Кнорре http://yeastmolbiol.belozersky.msu.ru. - why what you are studying is interesting/important.

Download Presentation

семинары “molecular biology of the cell”

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. семинары “molecular biology of the cell” Федор Северин, корпус А, комн. 220 тел. 939-31-07 8-915-120-7955 E-mail: severin@belozersky.msu.ru Святослав Соколов, Дмитрий Кнорре http://yeastmolbiol.belozersky.msu.ru

  2. - why what you are studying is interesting/important

  3. - why what you are studying is interesting/important - what is already known

  4. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions

  5. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • - motivation of a particular experiment

  6. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup

  7. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment

  8. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions

  9. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions

  10. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions • General conclusions, models, etc.

  11. motivation of a particular experiment • general experimental setup • specific experiment • Conclusions • Scc1’s cleavage and dissociation from chromosomes • depends both in vitro and in vivo on a protein called • Esp1. Previous studies have left unanswered whether separin • is itself the protease that cleaves Scc1 • To establish whether Scc1 can serve as a cleavage sub • strate whwe purified Scc1 from insect cells exp • pressing a Flag-epitope-tagged yeast SCC1 gene from • a recombinant baculovirus. • all Scc1-P was cleaved by extracts containing • intermediate levels of Esp1 separin (Figure 2B) • Thus, Esp1 separin might indeed be the protease that cleaves Scc1. However, we cannot elimiinnate at this stage the possibility that the Scc1 cleavage • activity is due to a substoichiometric protease that copurifies with separin. Uhlmann et al., Cell, 2000

  12. Актуальность предмета исследования Что известно

  13. Актуальность предмета исследования Что известно Мотивация исследования: вопросы

  14. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента

  15. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента

  16. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента

  17. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента Выводы по результату эксперимента

  18. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента Выводы по результату эксперимента

  19. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента Выводы по результату эксперимента Общие выводы, модели, гипотезы

More Related