DISCARD PROCEDURES

1. DISCARD PROCEDURES • Slides and broken glass are discarded into the cardboard box • Petri-dishes are placed in the transparent auto-clave bags • Tips/swabs/and tongue depressors will be discarded in the auto-clavebags • Only paper trash that has been contaminated with organism should go to the autoclave bag. Dispose all other paper trash in the garbage cans • DO NOT discard materials or trash in the satin troughs. If an item ( pen, slide, clothespin, etc., ) falls into the trough, retrieve it. • Leave only unused media/ supplies on the top of the cart.

2. Laboratory Rules • Food, drinks, and chewing gum are NOT allowed in lab. Nothing should go in into your mouth while you are in lab. • Long Hair must be tied back. Sandals are not encouraged. • The instructor must be informed of injuries and spills immediately • The lab bench must be cleaned with disinfectant and paper towels at the beginning and end of lab. Allow it to air dry. • Only notebook, lab manual, or writing utensil should be on the bench. Purses, backpacks etc., must be under the bench (or side bench) • Bunsen burners must be “OFF” when not in use

3. Requirements • Each student should bring to lab • Lab Manual • Lab Coat • A Sharpie (Marker, any color) • And most important is not to forget to bring yourself too

4. Petri dish Incubation WRONG CORRECT

5. Hand Washing • Prevents the spread of infection • Most microrganisms play beneficial roles in the health of a person • Normal Flora: • Inhibit pathogenic organisms • Antibiotics inhibit the growth of normal flora resulting in susceptibility to infections • Can be pathogenic due to changes in the environment such as pH changes and decease in immune function

6. It is found on the skin, mouth, intestine etc. • The skin is not favorable environement for microbial growth because • It is subject to periodic drying • Has an acidic pH of 4-6 • TRANSIENT FLORA • Organisms that may be present in a region but are not adapted and do not multiply there

7. Whasing hands serves: • Sloughs of the dead aoutermost layers of the skin • Removes transient flora • May expose normal flora • Prevents nosocomial infections (I.e., hospital aquired infections)

8. Procedure 1. Hand Washing • Before any hand washing , gently make a five finger impression on half of the plate • Wash your hand (2 minutes) with soap andmake another five-finger impressions (using the same and and fingers) on the second half of th eplate • Mark plate with your name, date and incubate @ 37 C (inverted)

9. Environmental sampling • Choose any 3 environmental surfaces such as a floor wall, table, drinking fountain, car keys, door handles, toilets etc. (BE CREATIVE) • Inoculate plate (Swab) back and forth the entire surface area of the TSA plate

10. Isolation of Normal Microbiota from human body • Skin • Take a swab, moisten it in 0.9% saline • Roll it over your arm or his/her arm • Swab a TSA plate • Throat • One student will swab his or her partner’s throat around the tonsillar area using a sterile cotton swab • Swab back and forth on edge of a blood agar plate or (TSA plate), then streak from this arae with a sterile loop.

11. Lab work • Hand washing • 1 TSA plate / Pair • Environment samples • 3 TSA plates / pair • Skin sample • 1 TSA plate / pair • Throat • 1 Blood Agar plate / pair before after