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CS (Soft)

Puroindolines ‘ a ’ and ‘ b ’ are completely linked with hardness. Puroindoline a. Puroindoline b. Absence of this gene is always associated to hardness. Presence of the PCR band for the “soft” allele combined with presence of the puroindolin a is characteristic of a soft wheats.

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CS (Soft)

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  1. Puroindolines ‘a’ and ‘b’ are completely linked with hardness Puroindolinea Puroindoline b • Absence of this gene is always • associated to hardness • Presence of the PCR band for the “soft” allele combined with presence of the puroindolin a is characteristic of a soft wheats • Absence is always associated with • a puroindoline b mutation present • in soft wheats BC1 Express / 2*UC1036 BC1 Express / 2*UC1036 BC1 Express / 2*UC1036 BC1 Express / 2*UC1036 Yecora Rojo (Hard) Cheyenne (Hard) Express (Hard) UC1036 (Hard) Yecora Rojo (Hard) CS (Soft) Express(Hard) UC1041 (Hard) UC1036 (Hard) UC1037 (Hard) Anza (Hard) RSI5 (Hard) CS (Soft) Hae III- MTA9 RFLP Puroindoline a PCR of puroindoline b sequence for the “soft” allele (Giroux & Morris TAG, 1997)

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