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Molecular Microbiology

Molecular Microbiology. Genetic Recombination. Vertical Binary fission Budding Horizontal Conjugation Transduction Transformation. Conjugation. Transduction. Transformation. Griffith Experiment. Biotechnology. Recombinant DNA Genetic Engineering Disease treatments SCID SSA CF

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Molecular Microbiology

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  1. Molecular Microbiology

  2. Genetic Recombination • Vertical • Binary fission • Budding • Horizontal • Conjugation • Transduction • Transformation

  3. Conjugation

  4. Transduction

  5. Transformation

  6. Griffith Experiment

  7. Biotechnology • Recombinant DNA • Genetic Engineering • Disease treatments • SCID • SSA • CF • Make products • Vaccines • Enzymes • Insulin • Hormones

  8. Plasmids • DNA • Replicate independently • Genetic Vectors • Small • Stable • Marker inserted • Gene expression

  9. Background • Donor • Recipient • Competent Cell • Natural • Change cell wall • Change PM • Artificial • Temperature • Salts (CaCl2) • Marker • Cloning Site

  10. LB Broth and Agar • Lysogeny Broth • Luria Broth • Luria-Bertani • Nutritionally Rich • Peptides, casein • Vitamins • Minerals (NaCl) • Trace elements • Biotech • Cultivate E.coli

  11. Procedure • Follow lab manual!! • Reader • Directs • Keeps time • Materials • LB plates • 2 types • LB • LB-amp • 2 situations • Before • After • Label, Label, Label • pGreen plasmid • CaCl2 • Micropipets • Spreader • Loop inoculation • Temperature • Incubator • Baths • Ice • Heat

  12. Results • Marker • Fluorescence • Cloning site • R-ampicillin • Colony observation • Natural light • UV light • Calculate transformation efficiency

  13. Questions? Glow Fish

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