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Part II . Protein Dynamics

Part II . Protein Dynamics. Chapter 14 Enzyme Kinetics. 14.1 Enzymes are Biological Catalyst. 1878 ------------ enzyme 1913 ------------ Michailis-Menten equation 1926 ------------ urease 1982 ------------ ribozyme 1986 ------------ abzyme.

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Part II . Protein Dynamics

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  1. Part II . Protein Dynamics

  2. Chapter 14 Enzyme Kinetics

  3. 14.1 Enzymes are Biological Catalyst 1878 ------------ enzyme 1913 ------------ Michailis-Menten equation 1926 ------------ urease 1982 ------------ ribozyme 1986 ------------ abzyme

  4. Nearly all enzymes are proteins, although a few catalytically active RNA molecules have been identified. Enzyme are catalysts that increase the rate of a chemical reaction without being changed themselves in the process.

  5. enormous catalytic power 8 20 10 ----10 • specificity substrate • regulation

  6. Holoenzyme = apoenzyme + cofactor Coenzyme Prosthetic group • Monomeric enzyme • Oligomeric enzyme • Multienzyme system

  7. 14.2 Enzyme Classification and Nomenclature Oxidoreductases Transferases Hydrolases Lyases Isomerases Ligases

  8. ATP:D-glucose-6-phosphotransferase hexokinase enzyme commision E.C.2.7.1.2 class Individual entry subclass sub-subclass

  9. 14.3 Kinetics of Enzymes-Catalyzed Reactions

  10. Substrate saturation curve for an enzyme-catalyzed reaction

  11. 14.3.1 The Michaelis-Menten Equation Enzyme-substrate complex k1 k3 k2 E + S ES E + P

  12. Michaelis-Menten Equation Vmax[S] v = ------------- Km + [S] • Constant Km V = Vmax / 2 , Km = [S]

  13. Lineweaver-Burk double-reciprocal plot

  14. 14.3.2 Effect of PH on Enzymatic Activity

  15. 14.3.3 Effect of Temperature on Enzymatic Activity Optimum temperature

  16. 14.4 Enzyme Inhibition Any molecule which acts directly on an enzyme to lower its catalytic rate is called an inhibitor.

  17. 14.4.1 Irreversible Inhibition Covalent bond

  18. 14.4.2 Reversible Inhibition • competitive inhibition

  19. noncompetitive inhibition

  20. 14.5 Enzyme Activity • Reactive velocity • Active unit (U) • Specific activity • U/mg protein • Turnover Number (kcat )

  21. 14.6 RNA and Antibody Molecules as Enzymes 14.6.1 Catalytic RNA Molecules: Ribozymes

  22. 14.6.2 Catalytic Antibodies: Abzymes

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