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Blood

Blood. Start. TABLE OF CONTENTS. Blood Components. Blood Typing. Blood Cell Differential Identification. Blood Cell Counting Using a Hemocytometer. Blood. BLOOD TYPING. ABO Agglutination Result. Rh Agglutination Result. Type A. Type AB. Rh +. Type B. Type O. Rh -.

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Blood

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  1. Blood Start

  2. TABLE OF CONTENTS Blood Components Blood Typing Blood Cell Differential Identification Blood Cell Counting Using a Hemocytometer Blood

  3. BLOOD TYPING ABO Agglutination Result Rh Agglutination Result Type A Type AB Rh + Type B Type O Rh - Antigen – Antibody Composition Blood Type Compatibility Comparisons Blood

  4. ABO Blood Typing Type A Type B Type AB Type O Blood

  5. Type A Agglutination Result Agglutination Note the appearance of fine granulation (pepper grains) in the blood No Agglutination Note the lack of granulation in the blood Blood

  6. Type B Agglutination Result No Agglutination Agglutination Blood

  7. Type AB Agglutination Result Agglutination Agglutination Blood

  8. Type O Agglutination Result No Agglutination No Agglutination Blood

  9. Rh Blood Typing Rh+ Rh- Blood

  10. Rh+ Agglutination Result Agglutination Blood

  11. Rh- Agglutination Result No Agglutination Blood

  12. BLOOD CELL DIFFERENTIAL IDENTIFICATION Human Blood Smear 400x Erythrocytes 400x 1000x Leukocytes Cell Types Thrombocytes 400x 1000x Blood

  13. Human Blood Smear 400x erythrocyte thrombocyte leukocyte Blood

  14. Erythrocytes 400x Blood

  15. Erythrocytes 1000x Blood

  16. LEUKOCYTES 1000x Neutrophil Eosinophil 1000x 1000x Basophil 1000x Lymphocyte Monocyte 1000x Blood

  17. Leukocyte - Neutrophil 1000x thrombocyte erythrocyte neutrophil Blood

  18. Leukocyte - Eosinophil 1000x erythrocyte eosinophil thrombocyte Blood

  19. Leukocyte - Basophil 1000x erythrocyte basophil thrombocyte Blood

  20. Leukocyte - Lymphocyte 1000x thrombocyte lymphocyte erythrocyte Blood

  21. Leukocyte - Monocyte 1000x erythrocyte monocyte thrombocyte Blood

  22. Thrombocytes 400x thrombocytes Blood

  23. Thrombocytes 1000x thrombocytes Blood

  24. BLOOD CELL COUNTING USING A HEMOCYTOMETER Microvolume Pipetters Hemocytometer Loading the Hemocytometer Hemocytometer Grid (Unloaded) 40x Hemocytometer Grid (Unloaded) 100x Hemocytometer Grid (Unloaded) 400x Hemocytometer Grid (Loaded) 100x Hemocytometer Grid (Loaded) 400x Blood

  25. Microvolume Pipetters Blood

  26. Hemocytometer grid grid Blood

  27. LoadingHemocytometer pipet tip cover slip Blood

  28. Hemocytometer Grid (Unloaded) 40x 1 2 3 4 5 RBC counted in 5 numbered areas Blood

  29. Hemocytometer Grid (Unloaded) 100x 3 Area 3 of previous magnification Blood

  30. Hemocytometer Grid (Unloaded) 400x Blood

  31. Hemocytometer Grid (Loaded) 100x Hemocytometer loaded with RBC Blood

  32. Hemocytometer Grid (Loaded) 400x inside of each box, cell not counted if on bottom line or on right line cell not counted cell counted inside of each box, cell counted if within box, on top line or on left line Blood

  33. separate from the lighter, liquid components (plasma) When whole blood is spun down in a centrifuge, the heavier, solid component (cells) … Blood

  34. click on a blood component Blood

  35. PLASMA (~55%) includes water, proteins, electrolytes, antibodies, organic nutrients and organic wastes Blood

  36. ERYTHROCYTES (~45%) Blood

  37. “BUFFY COAT” (~1%) LEUKOCYTES and THROMBOCYTES Blood

  38. ANTIGEN-ANTIBODY COMPOSITION TYPE A Rh+ TYPE B Rh- TYPE AB TYPE O Blood

  39. Antibody Antigen Antigen Type A Whole Blood Antibody A b RBC Blood

  40. Antibody Antigen Type B Whole Blood Antigen Antibody B a RBC Blood

  41. Antigens Antibodies Antigen Type AB Whole Blood A Antigen B RBC Blood

  42. Antigens Antibodies Antigen Type O Whole Blood Antibody B a Antibody b RBC Blood

  43. Antigen Antibody Antigen Rh+ Whole Blood Antibody Rh+ Antigen B a RBC Blood

  44. Antigen Antibody Antigen Rh- Whole Blood Antibody B a RBC Blood

  45. BLOOD TYPE COMPATIBILITY COMPARISONS Type A RBC Type B RBC Type A plasma Type AB plasma Type A plasma Type AB plasma Type B plasma Type O plasma Type B plasma Type O plasma Type AB RBC Type O RBC Type A plasma Type AB plasma Type A plasma Type AB plasma Type B plasma Type O plasma Type B plasma Type O plasma Blood

  46. Type A RBC Type A Plasma crossed with Antigen Antibody A b RBC Agglutination NO Hemolysis NO Compatible YES Blood

  47. Type A RBC Type B Plasma crossed with Antigen Antibody Antibody a a A RBC Agglutination YES Hemolysis YES Compatible NO Blood

  48. Type A RBC Type AB Plasma crossed with Antigen A RBC Agglutination NO Hemolysis NO Compatible YES Blood

  49. Type A RBC Type O Plasma crossed with Antigen Antibody Antibody a a A Antibody b RBC Agglutination YES Hemolysis YES Compatible NO Blood

  50. Type B RBC Type B Plasma crossed with Antibody Antigen Antibody a B a RBC Agglutination NO Hemolysis NO Compatible YES Blood

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