1 / 4

p18-/-

Primary cBMT. p18+/+. Long-term Engraftment. Secondary cBMT. p18-/-. Long-term engraftment. irradiated. p18+/+. irradiated. (re-challenged with equal amount of p18+/+ cells).

claraporter
Download Presentation

p18-/-

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Primary cBMT p18+/+ Long-term Engraftment Secondary cBMT p18-/- Long-term engraftment irradiated p18+/+ irradiated (re-challenged with equal amount of p18+/+ cells) • Semi-quantitative PCR analysis for ratios of the two competitive cell populations in bulk cells or different lineages based on relative intensities of the two genotypic bands on the gels; • Single cell or colony PCR for the absolute representation of each genotype in the stem or progenitor compartment based on the appearance of a distinct band on the gel. p18+/+ (600 bp) p18-/- (400 bp) Fig S1

  2. Fig S2a Sorting strategies for CD34-LKS cells R4 Propidium Iodide R1 Pulse Width Side Scatter Counts R2 R3 Forward Scatter Forward Scatter Forward Scatter Lineage-PE-Cy7 Gated in R1+R2+R3+R4 R5 Sca-1-PE c-Kit-APC Gated in R1+R2+R3+R4+R5 R6 Counts CD34-FITC

  3. Fig S2b 11 Gy p18+/+ 105 Sca-1- cells CD45.1/CD45.2 5-14 weeks CD45.1 Different doses of stem cells PE-CD45.1 p18-/- CD45.2 FITC-CD45.2

  4. Fig S3 Decreased apoptosis of p18 -/- donor cells was not observed 2 days following bone marrow transplantation Gated in CFSE+ cells Whole Lin- Lin+ 104 104 104 103 103 103 102 102 102 p18+/+ 9.93% 5.18% 4.52% 101 101 101 1.74% 0.37% 1.35% 100 100 100 100 101 102 103 104 100 101 102 103 104 100 101 102 103 104 104 104 104 103 103 103 p18-/- 102 102 102 14.41% 7.82% 6.25% 101 101 101 3.75% 0.87% 2.82% 100 100 100 DAPI 100 101 102 103 104 100 101 102 103 104 100 101 102 103 104 Annexin V

More Related