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pRE18

NotI. 8. 6. 6. 6. 7. 7. 7. 2. 2. 2. 3. 3. 3. 4. 4. 4. 5. 5. 5. 8. 8. 3’ 5’. 3’ 5’. NotI. NotI. 1. 5’ 3’. pRE18. T-A Ac. NotI. NotI. KpnI. SacI. KpnI. SacI. 1. 5’ 3’. 35S. 35S. hpt. polyA. c1300polyA35S. pRE18I1AcF(R). KpnI, SacI. KpnI, SacI.

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pRE18

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  1. NotI 8 6 6 6 7 7 7 2 2 2 3 3 3 4 4 4 5 5 5 8 8 3’ 5’ 3’ 5’ NotI NotI 1 5’ 3’ pRE18 T-A Ac NotI NotI KpnI SacI KpnI SacI 1 5’ 3’ 35S 35S hpt polyA c1300polyA35S pRE18I1AcF(R) KpnI, SacI KpnI, SacI RB LB 35S 35S 1 5’ 3’ hpt polyA 1F(R) Supplementary Figure 8 Schematic representation of constructing 1F and 1R.

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