1 / 4

Table S1

Table S1.

dara-dodson
Download Presentation

Table S1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Table S1 Table S1. The semi-conserved 7 nucleotide MEME motifof all selected and sequencedclones (30). The table includes the first 10 cloneswith the lowestp- value. The Initial start of the site is shown in the second column. The 10 clonesaregrouped in twosub-groupsindicated by the underlined 6 nucleotidefullyconservedsequencewithin the group.

  2. Table S2 Table S2. Rate constants calculated from Biacoresensograms of 400 nMRNA aptamers on amine coupled Protein E. 400 nMof each aptamer was injected at 10 µl/min on a Biacore CM5 chip with amine coupled Protein E (~1800 RU). The rate constants kobs and koffare the initial fast rates from a two-phase non-linear regression of 800 s of association and 800 s of dissociation. KD is calculated as koff/kon. For the rate constants shown in the table the R-value of the two-phase non-linear regression is shown below the rate value. The kofffor clone 1 and clone 23 that are underlined represents values with an R-value lower than 0.99 and no values could be obtained for clone 4 and the negative control d12.

  3. Figure S1 Figure S1. Thisfigure shows a 43 nucleotide long motifisolated from a MEME run on all 30 randomregions from the sequencedclones. The MEME motifanalysiswasrunwith no limitations on lenght. The presented motifwasfound in 11 clones.

  4. Figure S2 ―Clone 1 ―Clone 2 ―Clone 3 ― Clone 4 ― Clone 7 ― Clone 9 ― Clone 15 ― d12 Response (RU) Time (s) Figure S2. Binding of RNA aptamer on amine coupled PE in a Biacore. 400 nM of each aptamer was injected at 10 µl/min on a CM5 chip with amine-coupled PE (~1800 RU) on a Biacore 3000. The injection phase was 900 s and the dissociation phase was more than 1000 s. Data are Y-normalizedusing the Biaevaluation software.

More Related