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化脓菌分离鉴定过程(三)

化脓菌分离鉴定过程(三). 化脓菌分离鉴定过程. 葡萄球菌 甘露醇发酵试验 血浆凝固酶试验 耐热核酸酶试验 动物试验. 甘露醇发酵试验. Inoculate the bacteria into a mannitol micro-tube Incubate at 37℃ for 18h. S.aureus will ferment mannitol to produce acid, which causes the medium to turn yellow. 纤维蛋白原. 协同因子. 血浆凝固酶试验( The Coagulase Test ).

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化脓菌分离鉴定过程(三)

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  1. 化脓菌分离鉴定过程(三)

  2. 化脓菌分离鉴定过程 • 葡萄球菌 • 甘露醇发酵试验 • 血浆凝固酶试验 • 耐热核酸酶试验 • 动物试验

  3. 甘露醇发酵试验 • Inoculate the bacteria into a mannitol micro-tube • Incubate at 37℃ for 18h. • S.aureus will ferment mannitol to produce acid, which causes the medium to turn yellow.

  4. 纤维蛋白原 协同因子 血浆凝固酶试验(The Coagulase Test) • Coagulase • 结合凝固酶 • 游离凝固酶 • 凝固酶凝血酶样物质 纤维蛋白 • It has been speculated that this enzyme might be a virulence factor. However, coagulase-negative strains are often as pathogenic as coagulase-positive strains. • There are two ways of performing this test • The Slide Coagulase Test • The Tube coagulase Test

  5. + Tube coagulase Test

  6. The Slide Coagulase Test

  7. 细菌的药物敏感性试验 • 纸片扩散法(K-B法)

  8. 肠道菌分离鉴定过程(三)

  9. Antigens of salmonella O antigen polysaccharide of LPS used to divide Salmonella to 42 groups (A-Z,O51-63,O65-67) A-E groups are pathogenic H antigen flagella antigen divide Salmonella tow phase: special no special Vi antigen related to the virulence of Salmonella sensitive to heat ( lose activity at 60℃)

  10. Serological Identification of Salmonella Select the specimen React with A-Z polyvalent antiserum Agglutination reaction (+) (-) React with several individual types of anti-O and anti-H antiserum React with anti-Vi antiserum Identify its group and phage

  11. 系统实验报告(报告三) 化脓菌/肠道菌分离鉴定过程 目的 策略(原理设计) 方法步骤:简单写 结果(判定方法) 结论

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