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Frontal Analysis or Displacement

Frontal Analysis or Displacement. Frontal Analysis Concepts Continuously add sample to front of column Monitor components as they elute (exit from column) Provides a general measure of how compounds are retained on column Example - Charcoal flitration. Frontal Analysis Process

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Frontal Analysis or Displacement

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  1. Frontal Analysis or Displacement • Frontal Analysis Concepts • Continuously add sample to front of column • Monitor components as they elute (exit from column) • Provides a general measure of how compounds are retained on column • Example - Charcoal flitration • Frontal Analysis Process • Materials (solute) move down a column of packed material by being displaced by a more highly retained solute

  2. Partitioning

  3. Theory of Partitioning

  4. W1/2 W

  5. Selectivity Factor A B Resolution Retention Factor

  6. Does N depend on the order of elution? Detector response time

  7. A = B/u = C*u =

  8. What’s the optimum mobile phase velocity? When an acceptable level of separation is achieved, what choice does the experimenter have regarding optimum mobile phase velocity?

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