1 / 13

Characterizing spikes

Characterizing spikes. 8/11/2006. Jason Gilder. Spikes. Caused by voltage fluctuations and particles in the polymer Usually appear in three or more channels Usually appear at precisely the same data collection point. ABI literature. More ABI literature. Data set. 89 Samples

drake
Download Presentation

Characterizing spikes

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Characterizing spikes 8/11/2006 Jason Gilder

  2. Spikes • Caused by voltage fluctuations and particles in the polymer • Usually appear in three or more channels • Usually appear at precisely the same data collection point

  3. ABI literature

  4. More ABI literature

  5. Data set • 89 Samples • Reference Samples • Positive Controls • Negative Controls • 1010 “Good” peaks • All below 4000 RFUs • 55 peaks associated with 24 spike events

  6. Observed patterns

  7. Spike magnitude by dye

  8. Spike measurement Ratio = peak area / peak height

  9. Plot of peaks

  10. Results

  11. Establishing boundaries • We can capture 95% of the population with two standard deviations • Determine limits for observed ratios • Lower limit for “Good” peaks: 5.5 • Upper limit for spikes: 4

  12. 95% boundaries

More Related