1 / 22

The Effects of Sports Drinks on E.coli Survivorship

This study investigates whether sports drinks, specifically Gatorade, have adverse effects on the survivorship of E. coli bacteria. The experiment examines the growth and colony count of E. coli in different concentrations of Gatorade over varying exposure times. The results suggest that increased concentrations of Gatorade can lower the amount of E. coli cells that grow, while higher exposure times also contribute to decreased survivorship. However, further studies are needed to explore different types of sports drinks and test other types of cells. The study provides insights into the potential effects of sports drinks on microflora cells.

dsimons
Download Presentation

The Effects of Sports Drinks on E.coli Survivorship

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. The Effects of Sports Drinks on E.coli Survivorship Jonathan Farkos Grade 9 Pittsburgh Central Catholic High School

  2. Problem • Do sports drinks have adverse effects on microflora cells?

  3. Human Symbionts, Pathogens, and Flora • More than 10% of your body mass is due to symbiontsand pathogens • Mostly Prokaryotic cells • Some may cause diseases • Also protect the body from foreign invaders • Reinforce immune system, prevent allergies, and produce vitamins • Lie within skin, saliva, and gastrointestinal tracts • Break down abnormally large nutrients

  4. E.Coli c600 • Used in many cell/biochemical investigations • Easy to manipulate in labs • Normally found in the intestines of animals. • Some are pathogenic, but most are harmless • Large and diverse group of gram(-) bacteria • Utilized in this study as a human cell model and as a model of pathogens • E.coli was chosen for this experiment because of it’s diversity, it lives in the intestinal tract of humans and many other mammals, and it serves as a common prokaryotic cell model.

  5. Gatorade • Very commonly used beverage amongst athletes and non-athletes • Provides electrolytes • Common uses include: During sports, Post-sport recovery, and as a common drink • Thought to be non-toxic substance with no effects on body Ingredients: Contains water, sucrose (table sugar), dextrose, citric acid, natural flavor, sodium chloride (table salt), sodium citrate, monopotassium phosphate, and flavoring/coloring ingredients, and sucrose acetate isobutyrate.

  6. Purpose • To assess the effects of Gatorade on the survivorship of E.coli • The human micro flora performs many functions vital  to human health.

  7. Hypotheses Null Hypothesis: Gatorade will not affect the survivorship of E.coli Alternative Hypothesis: Gatorade will significantly reduce the survivorship of E.coli

  8. Materials • YEPD agar plates(1% Yeast Extract, 2% peptone, 2% dextrose) • Sterile pipette tips • Micropipettes • Vortex • Incubator • Spreading turntable • Spreader bar • Sports Drink (Gatorade) • Sterile capped test tubes with Sterile Dilution Fluid [SDF] (10mM KH2PO4, 10mM K2HPO4, 1mM MgSO4, .1mM CaCl2, 100mM NaCl) • E.Coli C600

  9. Procedure 1.Bacteria (E.coli) were grown overnight in sterile LB media. 2. Samples of the overnight cultures were added to fresh media in a sterile sidearm flask. 3. The cultures were placed in an incubator (37˚C) until a density of 50 Klett spectrophotometer units was reached. This represents a cell density of approximately 10^8 cells/mL. 4. The cultures were diluted in sterile fluid to a concentration of approximately 10^5 cells/mL. 5: LB agar plates(1% tryptone, .5% yeast extract, 1%NaCl) were prepared 6: Gatorade was pipetted into tubes containing SDF, to create the following concentrations: 0%, 1%, 10%, 25%, and 50%, yielding a final volume of 10 mL.

  10. Procedure (Continued) CHANGE The cells were vortexed for even suspension. 8. After vortexing to evenly suspend cells, 0.1mL aliquots were removed from the tubes at time 10 minutes and 50 minutes and spread on YEPD agar plates (5 plates per concentration)

  11. Procedure (Continued) • The plates were then incubated at 30 degrees for 48 hours. • The resulting colonies were counted. Each colony is assumed to have arisen from one cell.

  12. P Value- Less than 1x10E-6 P Value- 0.000528

  13. Dunnett’s Test T-Critical = 3.48

  14. Analysis of Exposure Time Did the duration of exposure have a significant effect?

  15. Conclusions • Generally, increased concentrations of Gatorade lowered the amount of E.coli cells that grew while some concentrations provided aid to growth • Increased exposure did have a significant effect on the amount of surviving colonies. • Higher concentrations of Gatorade could also be effective in reducing the amount of E.coli colonies that could survive

  16. Limitations • Limited number of concentrations • Only 1 species test • Only 2 exposure times • During the testing, plating could have possibly been unsynchronized. • Only one type of Sports Drink was used.

  17. Further Studies • Different types of Sports Drinks • More of a variety of concentrations • Experiment on other types of cells

  18. Sources • https://www.cdc.gov/ecoli/general/ • http://www.webmd.com/a-to-z-guides/tc/e-coli-infection-topic-overview#1 • https://microbewiki.kenyon.edu/index.php/Escherichia_coli • http://www.healthline.com/health/food-nutrition/is-gatorade-bad-for-you#1 • https://sites.psu.edu/siowfa15/2015/09/15/the-science-behind-gatorade/

  19. Data Results

  20. Data Results (Continued)

  21. Survivorship of E.coli

  22. Data Analysis (ANOVA)

More Related