1 / 23

Chromatography 研習資料

Chromatography 研習資料. Separation of Pigments In a solvent according To their densities. Separate plant pigments. Also for Forensics. Find type of ink (different color separations) Find impurities in products. Taper Strip. Cut to a point with scissors. Mark a starting line. Use pencil

dustin-carlson
Download Presentation

Chromatography 研習資料

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chromatography研習資料 Separation of Pigments In a solvent according To their densities

  2. Separate plant pigments

  3. Also for Forensics • Find type of ink (different color separations) • Find impurities in products

  4. Taper Strip • Cut to a point with scissors

  5. Mark a starting line • Use pencil • (not ink)

  6. Chemical Application Add small drops, let dry, add again until dark spot KEEP ABOVE PENCIL LINE

  7. Use coin (mechanical) – geranium leaf • Use serrated coin • Do a few times to make a dark line

  8. Be sure liquid is below line • Attach paper to paper clip • Attach paper clip to stopper

  9. Chromatography Animation Paper Chromatography Videos

  10. Chromatography Over Time • Time Lapse Video • (Be patient and give it time to move)

  11. Mark bands of colors Lighter pigments will move farther up the paper Heavier pigments will remain near the start

  12. Pencil line will mark start point

  13. Separation of Ink

  14. Calculating Rf • Compares distance the solvent traveled to the distance the test sample traveled. • Rf = distance of test sample • distance solvent

  15. Measure tops of colors • From starting point at bottom to top of each color Distance of solvent

  16. Pigments in Plants • carotene-yellow orange • chlorophyll a-bright green • Xanthophylls-yellow • chlorophyll b-khaki green

  17. More colors • phycocyanin = bluish • phycoerythrin = reddish

  18. Carrier Solution • Stored in da hood! • Return the waste to the waste container in da hood. • Boy-e-e-e-e-e-e-e-e-e-e!

  19. Sample Results

  20. Click Here for Examples • Results of Chromatograph

  21. Accessory Pigments • fucoxanthin = brown • Phycobilin = reds • phycocyanin = bluish

  22. Conclusion Question #2

More Related