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Intersubject Normalization for Group Analyses in fMRI

Intersubject Normalization for Group Analyses in fMRI. Jody Culham Brain and Mind Institute Department of Psychology University of Western Ontario. http://www.fmri4newbies.com/. Last Update: January 18, 2012 Last Course: Psychology 9223, W2010, University of Western Ontario.

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Intersubject Normalization for Group Analyses in fMRI

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  1. Intersubject Normalizationfor Group Analyses in fMRI Jody Culham Brain and Mind Institute Department of Psychology University of Western Ontario http://www.fmri4newbies.com/ Last Update: January 18, 2012 Last Course: Psychology 9223, W2010, University of Western Ontario

  2. Brains are Heterogeneous Slide from Duke course

  3. How can we define regions? Talairach coordinates • Example: The FFA is at x = 40, y = -55, z = -10 • Anatomical localization • Example: The FFA is in the right fusiform gyrus at the level of the occipitotemporal junction • Functional localization • Example: The FFA includes all voxels around the fusiform gyrus that are activated by the comparison between faces and objects Kanwisher, McDermott & Chun, 1997, J Neurosci

  4. Talairach Coordinate System • Talairach & Tournoux, 1988 • made an atlas based on one brain • any brain can be squished or stretched to fit hers and locations can be described using a 3D coordinate system (x, y, z) … from an alcoholic old lady Note: That’s TalAIRach, not TAILarach!

  5. Corpus Callosum Fornix Pineal Body “bent asparagus” Rotate brain into ACPC plane Find anterior commisure (AC) Find posterior commisure (PC) ACPC line = horizontal axis Note: official Tal says to use top of AC and bottom of PC but I suspect few people actually do this Source: Duvernoy, 1999

  6. Squish or stretch brain to fit in “shoebox” of Tal system z y<0 AC=0 y>0 y y>0 ACPC=0 y<0 x Deform brain into Talairach space • Mark 8 points in the brain: • anterior commisure • posterior commisure • front • back • top • bottom (of temporal lobe) • left • right Extract 3 coordinates

  7. Talairach Daemon • http://www.talairach.org

  8. Talairach Tables Source: Culham et al., 2003, Exp. Brain Res. • Talairach coordinates can be useful for other people to check whether their activation foci are similar to yours • Often it’s easiest to just put coordinates in a table to avoid cluttering text

  9. Variability between Japanese and European brains, both male (red > yellow > green > blue) Variability between male and female brains, both European (red > yellow > green > blue) Source: Zilles et al., 2001, NeuroImage Do We need a “Tarailach Atras”?

  10. MNI Space • Researchers at the Montreal Neurological Institute created a better template based on a morphed average of hundreds of brains (not just one brain like Talairach) • The MNI brain is more representative of average brain shape; however, it does not provide Brodmann areas • The MNI alignment is more complex than Talairach: SPM uses it but many software packages still use Talairach • CAVEAT: The MNI and Talairach coordinate are similar but not identical -- careful comparison requires a transformation -- converters can be found online http://brainmap.org/icbm2tal/index.html Source: http://www.mrc-cbu.cam.ac.uk/personal/matthew.brett/abstracts/MNITal/mniposter.pdf

  11. Another Look at RFX Huettel, Song & McCarthy, 2008

  12. Smoothing and Averaging Large activations across multiple subjects are more likely to show common activation than small ones --> Need to smooth (esp. for RFX analyses) UNDER CONSTRUCTION: Need to make a slide that shows this with real data

  13. Talairach Pros and Cons • Advantages • widespread system • allows averaging of fMRI data between subjects • allows researchers to compare activation foci • relatively easy to use • Disadvantages • not appropriate for all brains (e.g., Japanese brains don’t fit well) • activation foci can vary considerably – other landmarks like sulci may be more reliable

  14. Neurologic (i.e. sensible) convention • left is left, right is right L R - + x = 0 • Radiologic (i.e. stupid) convention • left is right, right is left R L + - x = 0 Left is what?!!! Note: Make sure you know what your magnet and software are doing before publishing left/right info! Note: If you’re really unsure which side is which, tape a vitamin E capsule to the one side of the subject’s head. It will show up on the anatomical image. (Remember which side you put it on!)

  15. Brodmann’s Areas Brodmann (1905): Based on cytoarchitectonics: study of differences in cortical layers between areas Most common delineation of cortical areas More recent schemes subdivide Brodmann’s areas into many smaller regions Monkey and human Brodmann’s areas not necessarily homologous

  16. gray matter (dendrites & synapses) GYRUS white matter (axons) BANK SULCUS FUNDUS Anatomical LocalizationSulci and Gyri pial surface gray/white border SULCUS FISSURE GYRUS Source: Ludwig & Klingler, 1956, in Tamraz & Comair, 2000

  17. Variability of Sulci Source: Szikla et al., 1977, in Tamraz & Comair, 2000

  18. Variability of Functional Areas • Watson et al., 1995 • - motion-selective area, MT+ (=V5) is quite variable in stereotaxic space • however, the area is quite consistent in its location relative to sulci • junction of inferior temporal sulcus and lateral occipital sulcus • see also Dumoulin et al., 2000

  19. Cortical Surfaces segment gray-white matter boundary render cortical surface inflate cortical surface sulci = concave = dark gray gyri = convex = light gray • Advantages • surfaces are topologically more accurate • alignment across sessions and experiments allows task comparisons

  20. Cortical Inflation Movie Movie: unfoldorig.mpeg http://cogsci.ucsd.edu/~sereno/unfoldorig.mpg Source: Marty Sereno’s web page

  21. Cortical Flattening 2) make cuts along the medial surface (Note, one cut typically goes along the fundus of the calcarine sulcus though in this example the cut was placed below) 1) inflate the brain 3) unfold the medial surface so the cortical surface lies flat 4) correct for the distortions so that the true cortical distances are preseved Source: Brain Voyager Getting Started Guide

  22. Spherical Averaging • Future directions of fMRI: Use cortical surface mapping coordinates • Inflate the brain into a sphere • Use sulci and/or functional areas to match subject’s data to template • Cite “latitude” & “longitude” of spherical coordinates Movie: brain2ellipse.mpeg http://cogsci.ucsd.edu/~sereno/coord1.mpg Source: Marty Sereno’s web page Source: Fischl et al., 1999

  23. Spherical Averaging Source: MIT HST583 online course notes

  24. Learning Brain Anatomy • Duvernoy, 1999, The Human Brain: Surface, Blood Supply, and Three-Dimensional Sectional Anatomy • beautiful pictures • good schematic diagrams • clear anatomy • slices of real brain • Springer, US$439 • Ono, 1990, Atlas of the Cerebral Sulci • great for showing intersubject variability • gives probabilities of configurations and stats on sulci • Theime, US$199 • Damasio,2005, Human Brain Anatomy in Computerized Images, 2nd edition • good for showing sulci across wide range of slice planes • 2nd edition much better than 1st edition • Oxford University Press, US$100 • Tamraz & Comair, 2000, Atlas of Regional Anatomy of the Brain Using MRI with Functional Correlations • good overview • Springer, US$203 • Talairach & Tournoux, 1988. Co-Planar Stereotaxic Atlas of the Human Brain • just because it’s the standard doesn’t mean it’s good (see also PC vs. Mac, VHS vs. betamax) • Theime, US$240 Wanna get rich? Publish a brain atlas. Sheesh, these are expensive!

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