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Other Detection System of Theorem Proving

Other Detection System of Theorem Proving. 2004. 2. 10 Park, Ji-Yoon. 1 × 3 Matrix. 15 mer. 15 mer. 45 mer. Poly-L-Lysine. 3 × 3 Matrix. 15 mer. 15 mer. 45 mer. 45 mer. 15 mer. 15 mer. 45 mer. 15 mer. 15 mer. 3 × 3 Matrix Chip (1). Experimental Steps.

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Other Detection System of Theorem Proving

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  1. Other Detection System of Theorem Proving 2004. 2. 10 Park, Ji-Yoon

  2. 1 × 3 Matrix 15 mer 15 mer 45 mer Poly-L-Lysine 3 × 3 Matrix 15 mer 15 mer 45 mer 45 mer 15 mer 15 mer 45 mer 15 mer 15 mer 3 × 3 Matrix Chip (1)

  3. Experimental Steps 1. Poly-L-Lysine Coating 4. Attachment of Target DNA 2. Attachment of Probe DNA 5. S1 nuclease Digestion 3. Washing & Dry 6. Washing & Dry 7. Confocal LIF Detection

  4. Amine-Coated Slide glass (Corning) Target DNA Cy5(R) Cy3(~P) FAM(ST~Q) Probe DNA ~RPQ ~T ~S

  5. Poly-L-Lysine Patterned Slide glass FAM Cy5 Overlay

  6. Problems & Discussion • PDMS Chip • Channel Cross-talking • Large sample volume require (~ 10 ul/channel) • We’ll use fresh PDMS Chip for every experiments • Using Thin PDMS Chip • Low Intensity of Cy3 • Cy 3 → Alexa fluoro dye (Molecular Probe)

  7. 5’ ST￢Q ￢P R ￢S ￢T QP￢R 5’ More Consideration Hybridization(6 strands) Ligation Surface Filtration Nil Detection S, T, Q, ￢P, R ￢S, ￢T, Q, P, ￢R

  8. 45 mer 15 mer 15 mer Nanoparticle-based Detection (2)Nature 382, 607-609 (1996)

  9. Au Experimental Step 1. Au modification with thiol-capped oligonucleotides - Note: Oligonucleotide: non-complementary 3-thiol-TACCGTTG 3’-thiol-TTTGCTGA Au 2. Assembly (700 nm: aggregation) - Addition of linking DNA duplex 5’-ATGGCAACTATACGCGCTAG 3’-ATATGCGCGATCTCAGCAAA 3. Immediately Color change - with naked eyes

  10. Expected Results (1) Fig 1. (A) The UV-vis spectrum of oligonucleotide-modified 13-nm particles before (red line) and after (blue line) DNA-induced assembly (B) Solutions of 13-nm-diameter Au particles before (cuvette A) and after (cuvette B) DNA-induced assembly (c) After extended periods of time a polymeric precipitate forms and settles to the bottom of the cuvette (cuvette C)

  11. Expected Results (2) Fig 2. TEM images of DNA-linked nanoparticle assemblies at early stages in the growth process

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