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Isolation and Identification of Spinach Pigments

Professor Matt And Colleen. Isolation and Identification of Spinach Pigments. Photosynthesis. Plants and organisms use for food Requires: Carbon Dioxide Water Sunlight Highly conjugated pigments Metal ions. Pigments. Highly conjugated proteins 4 types: Chlorophyll a Chlorophyll b

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Isolation and Identification of Spinach Pigments

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  1. Professor Matt And Colleen Isolation and Identification of Spinach Pigments

  2. Photosynthesis • Plants and organisms use for food • Requires: • Carbon Dioxide • Water • Sunlight • Highly conjugated pigments • Metal ions

  3. Pigments • Highly conjugated proteins • 4 types: • Chlorophyll a • Chlorophyll b • B-carotene • Xanthrophyllsviolaxanthin • Lutein • Structures provided in lab manual

  4. Chromatography • “color graphing” • Separation using intermolecular forces • Inter = between molecules • Capillary action • IMF strength due to: • Type • Shape • Size

  5. Rf value • Calculation using your measurements Distance moved by a compound, mm Distance moved by the solvent, mm

  6. Polarity • Based on: • Electronegativity • Molecular weight • Shape/orientation of molecules

  7. Groups for Absorbency • Group 1: 400-430, 600-640 • Group 2: 440-460, 650-690

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