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mCherry

G1. G2. Hoechst. GFP. Supp Figure 5. A. PEXEL. PBANKA_136550 327aa. mCherry. TM. B. pbanka_136550 ::mCherry. pbanka_136550 :: mCherry. ANKA wt. Chr 13 -. G1. G2. 95%(1%). anti- mCherry. ~100kDa. Hoechst. Chr 7 -. Uninfected rbc. ~70kDa. ctrl. 3’utr dhfr. mCherry. C.

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mCherry

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  1. G1 G2 Hoechst GFP Supp Figure 5 A PEXEL PBANKA_136550 327aa mCherry TM B pbanka_136550::mCherry pbanka_136550 ::mCherry ANKAwt Chr 13- G1 G2 95%(1%) anti- mCherry ~100kDa Hoechst Chr 7- Uninfected rbc ~70kDa ctrl 3’utr dhfr mCherry C pbanka_136550 ::mCherry schizont 20h schizont 24h ring 4h troph. 16h troph. 12h D Chr 13- Δ pbanka_ 136550 ANKAwt 1x107 5x106 ~1,5kb IBIS1 Chr 7- ctrl Chr 3- Δpbanka_136550 3’utr dhfr Δ pbanka_136550 K173cl1-GFP-Lucschiz -a Δ pbanka_136550 P<0.0001 P<0.0001 GFP+-schizonts (%) – G2 all schizonts (%) – G1 Hours post infection

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