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Introduction to Microscopy:

Introduction to Microscopy:. The Light Microscope The TEM The SEM. Introduction to Microscopy (Use of Microscopes to study minute [small] objects). The image to the right is a light microscope

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Introduction to Microscopy:

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  1. Introduction to Microscopy: • The Light Microscope • The TEM • The SEM

  2. Introduction to Microscopy (Use of Microscopes to study minute [small] objects) • The image to the right is a light microscope • Light microscopes bounce light waves off of an object. The light source may be from the sun or from a bulb from a lamp. • The diaphragm of a light microscope controls the amount of light the enters the stage to illuminate the specimen being examined.

  3. Resolution:The resolution of a microscope is a measure of the clarity the image produced by the scope; the smallest distance between to points that can be seen as being separate. • http://www.olympusmicro.com/primer/java/microscopy/airydiscs/index.html • The image below shows the difference between the resolution of a light microscope versus an electron microscope

  4. Electron Microscopes: these microscopes bounce a beam of electrons off of an object instead of light. These microscopes have a high resolution (smaller distance between two points)

  5. There are two types of electron microscopes: • SEM (scanning electron microscope) • Produces a three-dimensional image) • TEM (transmission electron microscope) • Produces a two-dimensional image)

  6. SEM Images (Three-Dimensional) http://micro.magnet.fsu.edu/primer/java/electronmicroscopy/magnify1/index.html

  7. TEM images

  8. Which has better resolution of image?

  9. Controls amount of light entering the stage

  10. http://images.google.com/imgres?imgurl=http://shs.westport.k12.ct.us/mjvl/biology/microscope/parts.gif&imgrefurl=http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm&h=314&w=458&sz=5&hl=en&start=1&um=1&tbnid=BLEnDPCBo0ZxeM:&tbnh=88&tbnw=128&prev=/images%3Fq%3Dhow%2Bto%2Buse%2Ba%2Bmicroscope%26svnum%3D10%26um%3D1%26hl%3Denhttp://images.google.com/imgres?imgurl=http://shs.westport.k12.ct.us/mjvl/biology/microscope/parts.gif&imgrefurl=http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm&h=314&w=458&sz=5&hl=en&start=1&um=1&tbnid=BLEnDPCBo0ZxeM:&tbnh=88&tbnw=128&prev=/images%3Fq%3Dhow%2Bto%2Buse%2Ba%2Bmicroscope%26svnum%3D10%26um%3D1%26hl%3Den

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