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Molecular Surveillance for Malaria Drug Resistant Genotypes in South America Kumar V. Udhayakumar, Ph.D Malaria Branch

Talk Outline. TrainingBackgroundPeru dataVenezuelan dataBrazilian data (Marinete Povoa)Diagnostics. Training. Ms. Nancy Arrospide VelascoInstituto Nacional de Salud (INS), PeruDates: 02/06/2008 to 04/30/2008Dr. Marinete M. Povoa, PhD Ms. Giselle Maria Rachid Viana, MScInstituo Evandro

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Molecular Surveillance for Malaria Drug Resistant Genotypes in South America Kumar V. Udhayakumar, Ph.D Malaria Branch

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    22. Copy Number Variation in pfMDR1 Mefloquine resistance has been linked to duplications of pfmdr1: More copies means more MQ resistance1-4 We used Real time PCR to test pfmdr1 copy number in 90 Venezuelan samples collected in between 2002-2004 All run at least in triplicate Samples with multiple copies of pfmdr1 were verified. 1 Wilson, et al., 1993, Mol. Biochem. Parasitol. 2Price et al., 1999, Antimicrob. Agents Chemother. 3Pickard et al., 2003, Antimicrob. Agents Chemother. 4 Price et al., 2004 Lancet

    28. Molecular Epidemiology • CDC collaboration • Objectives: Molecular surveillance for tracking the origins and spread of drug resistant mutations in Brazil and other parts of South America. Sequencing and microsatellite studies for: PfCRT dhfr dhps • Samples: 243 from different Brazilian Amazonia states collected in 80`s, 90`s, 2000`s

    34. PERSPECTIVES

    35. Pfcrt ANALYSES • Sequencing - presence of mutations in pfcrt (K76T) • Microsatellites – frequency and evolutionary history PfCRT (39) - 11 microsatellites

    36. PRELIMINAR RESULTS

    37. Team: Laboratório de Pesquisas Básicas em Malária (Parasites and Vectors)

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