1 / 25

HUMAN enterovirus AND VIRAL MENINGITIS IN SOUTHERN BRAZIL

HUMAN enterovirus AND VIRAL MENINGITIS IN SOUTHERN BRAZIL. Luine Rosele Renaud Vidal Universidade Federal do Paraná Financial Support : Secretaria de Ciência e Tecnologia do Estado do Paraná- SETI - PR. Research Background. Notifiable Disease in the N ational Health System

Download Presentation

HUMAN enterovirus AND VIRAL MENINGITIS IN SOUTHERN BRAZIL

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. HUMAN enterovirus AND VIRAL MENINGITIS IN SOUTHERN BRAZIL LuineRosele Renaud Vidal Universidade Federal do Paraná Financial Support: Secretaria de Ciência e Tecnologia do Estado do Paraná- SETI - PR

  2. Research Background • NotifiableDisease in theNational Health System • Around 1000 meningitis cases per year • Alletiology • Estimated 60% - viral etiology

  3. Research Background DistributionofViral Meningitisin Curitiba City byNeighborhood

  4. Research Background 460 CSF Samples July 2005 – December 2006 13% Positive

  5. Research Background Vidal et al, 2011

  6. Research Background

  7. Research Background

  8. Research Background • July 2005 – September 2008 • 1010 CSF samples (immunocompromised / immunocompetent) • 4.55% positive bynested PCR (nPCR) • 50% encephalitis • 40% meningoencephalitis • 2.5% mielitis Central NervousSystem (CNS) InfectionsbyHerpesviridaeFamily

  9. Research Background Central Nervous System (CNS) Infections by Herpesviridae Family %

  10. Molecular characterizationoFHuman enterovirus • July 2005 – December 2006 • 440 CSF samples – meningitispatients • 49 positive samples - 11%

  11. MeningitisPatients InclusionCriteria ExclusionCriteria EncephalitisParenquimaEnvolvement BacterialMeningitis Inadequatesample: collection / transport / storage • Acuteonset: < 4 weeks • Fever: > 38 °C • WBC: > 4 cell/mm3 • LymphocytesPredominance • Normal Glucose (≥ 45 mG/dL) • Lactate: < 3.5 mmol/L

  12. MeningitisPatients

  13. METHODS Casas et al, Journalof Medical Virology,1999 Obersteet al, Virus Research, 1999

  14. Seasonality

  15. Nucleotidesequencing EV Typing 12 samples

  16. Nucleotidesequencing HumanEchovirus 30

  17. Nucleotidesequencing ECHOVIRUS 4

  18. Nucleotidesequencing HUMAN ECHOVIRUS

  19. Conclusions • The molecular methodologywasefectiveandfastto define theetiologyofmeningitis. • CNS infection for EV wasobserved in thesummer (highertemperatures). • The EV typesfoundwereHumanEchovirus • It wasobserved a predominanceof EV infection in the ages of 4 to 17 years old. • It wasnotpossibletoidentifytheetiologicagent in themajorityofthesamples.

  20. Currently • Establishing a flowchart for CSF collecting • Introduction of lactate quantification • DNA/RNA Extraction – commercial reagentes • Real Time PCR Changesand Benefits

  21. Investigationand Molecular Epidemiology: • Adenoviruses • Flavivirus • Arenavirus • Patientswithsignsandsymptomsofencephalitisandmeningoencephalitis Future Researches

  22. Future Researches Human Parechovirus - HPeV Project CSF samplesstoredfrom2007-2014 Objectivetoestablishtheimpactofthevirus

  23. General Hospital Universidade Federal do Paraná

  24. Team VirologyLaboratory

  25. Curitiba City Bothanic Garden

More Related