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Histology

Histology. Microscopy used in this course. Light Microscopy ( LM ) – paraffin embedded, 7 micron sections, specific stains Transmission Electron Microscopy ( TEM ) – plastic embedded, 70 millimicron sections, heavy metals (electron-dense) stains

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Histology

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  1. Histology Microscopy used in this course

  2. Light Microscopy (LM) – paraffin embedded, 7 micron sections, specific stains • Transmission Electron Microscopy (TEM) – plastic embedded, 70 millimicron sections, heavy metals (electron-dense) stains • Scanning Electron Microscopy (SEM) – critically point dried, gold coated

  3. Light Microscopy

  4. TEM

  5. SEM

  6. Epithelium Simple Epithelium

  7. Simple Squamous Mesothelium

  8. Simple Squamous (Mesothelium) by TEM

  9. Simple Squamous – Endothelium (En)

  10. Simple Squamous – Endothelium (Ed) by TEM

  11. Capillary (endothelial cell) by TEM

  12. Simple Cuboidal Epithelium (longitudinal section)

  13. Simple Cuboidal (cross section)

  14. Simple Cuboidal by TEM

  15. Simple Columnar with Goblet Cells

  16. Simple Columnar (cross section) with Goblet Cells (arrow)

  17. Goblet Cell with PAS stain (red)

  18. Simple Columnar with Goblet Cells (Mu) by TEM

  19. Simple Columnar mucus secreting

  20. Simple Columnar mucus secreting

  21. Epithelium Stratified Epithelium

  22. Pseudostratified Ciliated Epithelium with Goblet Cells

  23. Pseudostratified Epithelium with Cilia and Goblet Cells (Respiratory Epithelium)

  24. Stratified Squamous non-Keratinized Epithelium

  25. Transitional Epithelium

  26. Transitional Epithelium with rounded binucleated surface cells

  27. Stratified Squamous Keratinized (thin)

  28. Stratified Squamous Keratinized (thick)

  29. Epithelial Cell Junctions

  30. Junctional Complexes

  31. Tight Junctions (Zonula Occludens) by TEM

  32. Tight Junctions (Zonula Occludens) by Freeze Fracture

  33. Tight Junction diagram

  34. Gap Junction (Nexus) by TEM

  35. Gap Junction 2 nM gap between membranes Connexin molecules Open pores for ions, molecules, etc

  36. TEM of Zonula Adherens (red arrows) TEM of a Desmosome (blue arrow)

  37. TEM (colorized) of Adherens Junctions

  38. TEM of a Desmosome with tonofibrils

  39. Desmosome Structure

  40. TEM of a Hemi-Desmosome

  41. Summary of the maintenance of cell shape by junctions and cytoskeletal elements

  42. Glandular Epithelium

  43. Serous Gland with ducts (arrows)

  44. Serous secretory unit (high magnification)

  45. Mucous Gland with duct (arrow)

  46. Mixed Gland with Serous Demilunes (SD)

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