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Determining the Iron Content in Vitamins

Determining the Iron Content in Vitamins. Rachel Warehime & John Siller. Purpose. Use two different methods Gravimetric determination Spectrometry determination Various vitamins One A Day (Women’s and Men’s) Prenatal Vitamins General Complete Multivitamin Kids Chewable Tablet

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Determining the Iron Content in Vitamins

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  1. Determining the Iron Content in Vitamins Rachel Warehime & John Siller

  2. Purpose • Use two different methods • Gravimetric determination • Spectrometry determination • Various vitamins • One A Day (Women’s and Men’s) • Prenatal Vitamins • General Complete Multivitamin • Kids Chewable Tablet • Senior Vitamins

  3. Procedure #1 • Fired crucibles and weighed until mass was reproduced. • Weigh out 0.5 gram sample of each vitamin • Dissolve in 3M HCl, add 5 mL 6M HNO3, boil for 2 minutes. • Dilute to 200 mL, add 3M ammonia until basic, and boil for 5 minutes. • Decant supernatant through ashless filter paper and wash the solid with 1 % NH4NO3. • Allow filter paper to dry for 24 hours. • Place filter paper with solid in weighed crucibles and ignite the paper in the 900◦C oven and put the lid on the crucible. • Let the crucibles cool for 15 minutes and then weigh them to determine the weight of iron left in the crucibles.

  4. Data • Men’s (A&G) • A = 0.5010 g G = 0.5022 g • Women’s (B&H) • B = 0.5010 g H = 0.5001 g • Senior (C&I) • C = 0.5040 g I = 0.5012 g • Kids (D&J) • D = 0.5008 g J = 0.5023 g • Prenatal (E&K) • E = 0.5019 g K = 0.5008 g • General (F&L) • F = 0.5022 g L = 0.5003 g

  5. Milligrams of Fe found in vitamins • Men’s (A&G) • A = 1.61 mg (0.0012 g) G = 4.13 mg (0.003 g) • Women’s (B&H) • B = 18.67 mg (0.0132 g) H = 18.05 mg (0.0129 g) • Senior (C&I) • C = 27.8 mg (0.02 g) I = 24.1 mg (0.0174 g) • Kids (D&J) • D = 8.8 mg (0.0063 g) J = 7.41 mg (0.0053 g) • Prenatal (E&K) • E = dropped lid K = 23.7 mg (0.017 g) • General (F&L) • F = 132.9 mg (0.0958 g) L = 214.5 mg (0.1534 g)

  6. Comparison • Men’s • Actual = 0 mg Experimental = 1.61 mg & 4.33 mg • Women’s • 18 mg Experimental = 18.67 mg & 18.05 mg • Senior • 8 mg Experimental = 27.8 mg & 24.1 mg • Kids • 15 mg Experimental = 8.8 mg & 7.41 mg • Prenatal • 28 mgExperimental = dropped lid & 23.7 mg • General • 18 mg Experimental = 132.9 mg & 214.5 mg

  7. Procedure #2 • Place one tablet in a flask with 25 mL of HCl and boil for 15 minutes. • Filter the solution once cooled and dilute 5 mL to 100 mL in flask. • From this solution, we made 4 more solutions. • Pipet 10 mL, 5 mL, 2 mL, and 1 mL into different flasks and dilute them all to 100 mL with distilled water. • Measure the absorbance of these solutions at a wavelength of 265 nm

  8. Data • Mass of tablets • Men’s 1.4974 g • Women’s 1.7850 g • Senior 2.2343 g • Kids 1.1538 g • Prenatal 1.1063 g • General 1.5455 g

  9. Absorbance Graphs

  10. Absorbance Graphs

  11. Absorbance Graphs

  12. Discussion • First procedure was successful • Second procedure was not • Human error • Better procedure • Not using certain chemicals listed • Were not available

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