1 / 11

Ultrasensitivity and noise propagation in a synthetic transcriptional cascade

Ultrasensitivity and noise propagation in a synthetic transcriptional cascade. Sara Hooshangi, Stephan Thiberge, and Ron Weiss Presented by John Chattaway, Christin Sander. Introduction. The paper investigated transcriptional cascades Noise Sensitivity Response Times

henry
Download Presentation

Ultrasensitivity and noise propagation in a synthetic transcriptional cascade

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Ultrasensitivity and noise propagation in a synthetic transcriptional cascade Sara Hooshangi, Stephan Thiberge, and Ron Weiss Presented by John Chattaway, Christin Sander

  2. Introduction • The paper investigated transcriptional cascades • Noise • Sensitivity • Response Times • Synchroniation/Cell-cell variability

  3. Inhibitory Cascades aTc: anhydrotetracycline

  4. Longer Transcriptional Cascades Have Increased Steady-State Sensitivity • Confirms previous theoretical studies • Sharper steady state transfer in longer cascades

  5. Response Times • Increase with cascade length • Increases length of time before circuit starts to react to stimulus • Circuit 1: 120min. • Circuit 2: 400min. (to reach steady-state) • Circuit 3: 600min.

  6. Cell–Cell Variability • Cell-cell variability increases with longer cascades • Noise in transition region is amplified as cascade becomes longer

  7. Synchronization of Cell Responses • Hardly any variation in Circuit 1 • Peak in variation during signal transduction in Circuit 3

  8. Low-Pass Filters 5min. 45min. 60min.

  9. Matching kinetic rates • Need to match kinetic rates of enzymes to output of gene • All genes in the system need to have similar rates of transcription and activation

  10. Effects in nature • Division • Stochastic Nature of reactions • Small number of molecules / longer cascades

  11. Questions & Answers

More Related