1 / 42

Molecular Luminescence Spectrometry

Molecular Luminescence Spectrometry. Fluorometer Evolution. Aminco-Bowman Spectrophotofluorometer. Quantum Yield related to rates:. Effects of Cell Geometry. Potential for “Inner-filter Effect”. b. a. Broad spectra are still a challenge when more than one element fluoresces

hong
Download Presentation

Molecular Luminescence Spectrometry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Molecular Luminescence Spectrometry

  2. Fluorometer Evolution

  3. Aminco-Bowman Spectrophotofluorometer

  4. Quantum Yield related to rates:

  5. Effects of Cell Geometry

  6. Potential for “Inner-filter Effect” b a

  7. Broad spectra are still a challenge when more than one element fluoresces Nevertheless, selectivity is much improved over absorbance.

  8. More spectral information assists in the analysis of mixtures

  9. Possible to choose a different path across the 3-d spectrum

  10. Gasoline engine exhaust analysis with a synchronous scan

  11. Phosphorescence Inhibit fluorescence and non-radiative decay 1) Freeze at low temperature 2) Immobilize on solid substrate 3) Dissolve in organized medium 4) Add a heavy atom Then isolate phosphorescence from fluorescence

  12. PABA immobilized on filter paper

  13. Perylene at 15K in A. Ethanol B. Hexane

  14. Perylene in hexane

  15. Narrow emission peaks at low temp.

More Related