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The Field-Flow Fractionation principle

Sample. introduction. External. field. The Field-Flow Fractionation principle. Length. Flow. Thickness. Breadth. Detector. Thickness. Sample. Parabolic flow. Some applications of FFF. particle diameter. 1 nm. 100 nm. 10 µm. ThFFF. ElFFF. SdFFF. GFFF. Polymeric beads.

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The Field-Flow Fractionation principle

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  1. Sample introduction External field The Field-Flow Fractionation principle Length Flow Thickness Breadth Detector Thickness Sample Parabolic flow

  2. Someapplications of FFF particlediameter 1 nm 100 nm 10 µm ThFFF ElFFF SdFFF GFFF Polymeric beads Bacteria, cells Inorganic colloids FlFFF Water soluble polymers Polymeric latexes Proteins, protein complexes Viruses 2 6 10 14 Log MW

  3. Standard channel for Flow FFF Longitudinal flow Inlet (Sample inlet) Longitudinal flow Outlet (to the detector) Cross-flow Inlet Plexiglas Top Bar Frit Spacer Membrane Frit Plexiglas Bottom Bar Cross-flow Outlet

  4. The HF FlFFF channel Flow Flow Field 1/8” PE fitting 1/8” PE Tee 1/8” Teflon tube cPVC / PSfHF membrane 24x0.08 ID cm 1/8” PEEK ferrule 1/8” PEEK ferrule

  5. 0.50 0.75 0.75 0.75 0.50 0.75 0.75 0.75 Data acquisition 2 2 1 1 3 3 0 0 0.123 Sample cell Detector The HF FlFFF instrumental scheme 4-way valve Metering valve Pressure gauge Pump 1 T-valve Waste Injection port Mobile phase Pump 2 Pressure gauge Hollow fiber channel Metering valve Mobile phase Waste

  6. < > v HF FlFFF in normal mode Brownianretention mechanism z L rf Flow l C(z) C0 x U Field

  7. Basic expressions in normal HF FlFFF Flow Field Retention

  8. Efficiency in HF FlFFF å = + + + + H H H H H H neq long poly inj i Focusing L0 Elution van Bruijnsvoort, M.; Kok, W.Th; Tijssen, R. Anal Chem 2001, 73, 4736

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