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Italy: tomato chr. 12 Country Representative: Dr. Giovanni Giuliano

Italy: tomato chr. 12 Country Representative: Dr. Giovanni Giuliano. Contribution from Naples Funding Agency : Italian Ministry of Agriculture (MiPAF) Project title : AGRO-NANOTECH Coordinator : Prof. Luigi Frusciante (UNIV. of Naples)

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Italy: tomato chr. 12 Country Representative: Dr. Giovanni Giuliano

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  1. Italy: tomato chr. 12 Country Representative: Dr. Giovanni Giuliano Contribution from Naples Funding Agency: Italian Ministry of Agriculture (MiPAF) Project title:AGRO-NANOTECH Coordinator: Prof. Luigi Frusciante (UNIV. of Naples) Investigators: Dr. Silvana Grandillo (IGV-CNR, Naples) Dr. Maria Raffaella Ercolano (UNIV. of Naples) Dr. Amalia Barone (UNIV. of Naples) Dr. Maria Luisa Chiusano (UNIV. of Naples) Total award for sequencing: 560,000 euro Start date: April 2004

  2. o Chromosome 12 seed BACs Overgo cM #BACs probes 12 TM14B TG180 2 11.0 T659 7.4 13 14.0 cLPT6E9 CT19A 1 19.0 TG263 6.4 1 21.0 TG68 Projected # of BACs 113 Euchromatin portion = 11Mb TG68 6 26.0 T1487 14.4 14 32.0 T1481 14 33.0 T0028 19 36.0 T0989 CT79 4.3 3 CT211A 40.0 T1667 22 5.5 41.0 cLET8K4 STATUS: Seed BACs to be sequenced are being selected from the available list of 250 seed BACs which were identified in Cornell, for chr. 12, by means of 30 OverGo probes (http://www.sgn.cornell.edu). CT219 2.2 52.0 T1736 12 TG360 0.8 52.0 T1211 10 CD6A 1.6 TG283 52.0 T1093 6 4.5 52.0 T1045 13 TG394 55.0 T1078 1 56.0 T1622 3 13.4 59.0 T1451 21 59.0 P62 17 CT287A 60.0 SSR44 4 60.0 SSR124 20 6.3 68.0 TG394 17 TG28A 1.0 CT239B 71.0 T1266 3 2.0 TG296 2.4 CT156 84.0 T1676 8 7.2 96.0 T1784 5 CT80B 96.0 TG296 2 6.1 97.0 T0882 3 CT276 101.0 T0770 6 5.8 TG473 110.0 T1504 1 1.8 CD2 110.0 T0800 1 120.0 CD2 2 249 30 markers

  3. 113 BACs ??? Available BACs 50 BACs 5 BACs Sequencing Service Center Local Bioinformatics Service (setup according to the Sol Genome Pipeline) Chromatogram (.abi,.scf) Phred scores Raw sequence (fasta) Structural and functional annotation Computational prediction Conflictsresolution and manual curation Re-sequencing Analysis Project specific analysis: Comparative genomics and Phylogenetics Identification and characterization of genes Identification of Molecular Markers SNP Microarray design …….. Project specific analysis: Comparative genomics Phylogenetics Miglioramento genetico ……..

  4. Facilities:  Biothecnological DNA sequencers (ABI 310, ABI 3100, ABI 3100avant) Real Time PCR Typhoon image scan  Computational Hardware: Servers (Silicon Graphics, SUN, IBM) Workstations (Linux, Windows XP, Mac/OS), Personal Computers Software: open source software for structure and functional analyses of Nucleic Acids and Proteins

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