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中華牙醫學會第三十二屆學術研討會 中華民國口腔顎顏面放射線學會九十八年度學術研討會

中華牙醫學會第三十二屆學術研討會 中華民國口腔顎顏面放射線學會九十八年度學術研討會. 陳玉昆， Y-K Chen Department of Oral Pathology, School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, TAIWAN. 虛擬顯微鏡於口腔顎顏面 病理學實驗課程之應用 An A pplication of V irtual M icroscopy to the

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中華牙醫學會第三十二屆學術研討會 中華民國口腔顎顏面放射線學會九十八年度學術研討會

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  1. 中華牙醫學會第三十二屆學術研討會 中華民國口腔顎顏面放射線學會九十八年度學術研討會 陳玉昆，Y-K Chen Department of Oral Pathology, School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, TAIWAN 虛擬顯微鏡於口腔顎顏面 病理學實驗課程之應用 An Application of Virtual Microscopy to the Teaching of Oral & Maxillofacial Pathology Laboratory Course 27th November, 2009

  2. 參 考 資 料 • Evans AJ, et al. Primary frozen section diagnosis by robotic microscopy and virtual slide telepathology: the University Health Network experience. Human Pathology 2009;40:1070-81. • Dee FR. Virtual microscopy in pathology education Human Pathol 2009;40: 1112-21. • Weinstein RS, et al. Overview of telepathology, virtual microscopy, and whole slide imaging: prospects for the future. Human Pathol 2009;40:1057-69. • Kaohsiung Medical University, Department of Oral Pathology. • Marchevsky AM, et al. Self-instructional “Virtual Pathology” laboratories using web-based technology enhance medical school teaching of pathology. Human Pathol 2003;34:423-9. • Chen YK, et al. An application of virtual microscopy to the teaching of oral and maxillofacial pathology laboratory course. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:342-7. • Hanks CT, et al. Internet- and DVD-based instruction in oral pathology for dental students. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;98:195. • Farah CS, et al. The e-evolution of microscopy in dental education. J Dental Edu 2009;73:942-9. • Dotslide, Olympus company catalogue

  3. Virtual microscopy: emulate traditional light microscopy using digital image files (virtual slides) manipulated on a computer screen using microscope emulator software Automate scan process - 50 slides Hard Copy 顯微影像數位掃瞄系統 Whole slide imaging: create digital images of the entire area of a glass slide Soft Copy Refs. 4, 9

  4. Virtual microscopy: use computer mouse to perform the control functions ordinarily handled with a traditional light microscope (position the objective lens) Ref. 4

  5. OlyVIA 閱片 Dot slide 掃瞄、閱片 Dot slide Procedures of whole slide scanning (1) Ref. 4

  6. New slide Dot slide Procedures of whole slide scanning (2) Ref. 4

  7. Overview image Dot slide Procedures of whole slide scanning (3) Ref. 4

  8. Dot slide Procedures of whole slide scanning (4) Ref. 4

  9. Edit scan area Dot slide Procedures of whole slide scanning (5) Ref. 4

  10. New area Dot slide Procedures of whole slide scanning (6) Ref. 4

  11. Live (active) Dot slide Procedures of whole slide scanning (7) Ref. 4

  12. Autofocus (AF) Live (active) Dot slide Procedures of whole slide scanning (8) Ref. 4

  13. Start Dot slide Procedures of whole slide scanning (9) Ref. 4

  14. Dot slide Procedures of whole slide scanning (10) Ref. 4

  15. Live (active) Dot slide Procedures of whole slide scanning (11) Ref. 4

  16. Dot slide Procedures of whole slide scanning (12) Ref. 4

  17. Save file Extended Focal Imaging (EFI) Dot slide Procedures of whole slide scanning (13) Ref. 4

  18. Dot slide Procedures of whole slide scanning (14) Extended Focal Imaging (EFI) Ref. 4

  19. Virtual slide viewer: use iconic representations of 4×, 10×, 20×, and 40× objective lenses and select the magnification of choice by clicking on one of these icons with a computer mouse OlyVIA Ref. 4

  20. Net Image Server/Web Server, Dotslide and OlyVIA Workstation Clients Dot slide/ OlyVIA Dot slide/ OlyVIA Net Image Server Dot slide/ OlyVIA Dot slide Dot slide/ OlyVIA Web Server Lan/Internet Refs. 4, 9

  21. Telepathology: practice of pathology at a long distance, has advanced continuously since 1986 Frozen Section Telepathology UHN Toronto Ontario Canada Robotic control of microscopy Ref. 1

  22. Virtual microscopy or telepathology - attractive to educators due to: (1) nearly perfectly simulates the pan & zoom characteristics of conventional microscopy (2) versatility of computer-assisted education Refs. 3, 8

  23. Medical schools in the US: entirely digital for pathology courses, discarding light microscopes, and building virtual slide laboratories UCLA School of Medicine’s Center Ref. 5

  24. UCLA School of Medicine’s Center Table 1. Mean Ratings of Attributes of Virtual Pathology Based on a Likert Scale (1, Not at All Useful to 5 Very Useful), n = 72 Ref. 5

  25. UCLA School of Medicine’s Center Table 2. Mean Ratings of Student Perceptions of Virtual Pathology Based on a Likert Scale (1, Not at All Useful to 5 Very Useful), n = 72 Ref. 5

  26. Oral Pathology Laboratory course for dental students at University of Michigan - converted from a microscope-based course to an internet-accessible virtual histopathology ‘‘laboratory.’’ (2003) Ref. 7 University of Queensland, School of Dentistry (Brisbane, Australia) implement VM technology (Aperio Systems’ImageScope) into its courses of Oral Biology (2006)& Oral Pathology(2008) Ref. 8

  27. 口腔病理學(含實驗) - KMUOP 1. 屬於基礎範圍，為提昇同學學習之興趣，於2006年開始應用網路及多媒體教學，架構口腔病理科教學網頁 2. 假設虛擬顯微鏡，將口腔病理教學玻璃切片轉換成高解析度的數位影像檔，置放於本科網站中。下載OlyVA軟體，使用寬頻網路閱覽口病教學切片。 Refs. 4, 6

  28. Ref. 4 Ref. 4

  29. Ref. 4 Ref. 4

  30. Ref. 4 Ref. 4

  31. Ref. 4

  32. Remote log in Ref. 4

  33. User name Password Log in administrator ………. Ref. 4

  34. Open remote file Ref. 4

  35. Search now Ref. 4

  36. Ref. 4

  37. Open Ref. 4

  38. Ref. 4

  39. Ref. 4

  40. Ref. 4

  41. 高醫牙科口腔病理學實驗切片線上教學 小組線上教學 發表文章： An application of virtual microscopy in the teaching of an oral and maxillofacial pathology laboratory course. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:342-7 線上考試 Refs. 4, 6

  42. (考試時間為50分鐘) 口病實驗期末考 • 內容： • 寫出口腔病理診斷 • 標示口腔病理特徵 (請務必寫上姓名與學號) Ref. 4

  43. (請務必詳細閱讀) 注意事項 • 點選OlyVIA後，工具列中點選remote login，鍵入個人帳號、個人密碼及IP Address:163.15.173.75，max.Hits:改為500、下載考試題目 • 考試當天，於口病網頁：oralpathol.dlearn.kmu.edu.tw下載考試用ppt檔於D槽，務必更改檔名為自己的姓名與學號，如 ’陳大明9512345’。(完成下載考試用ppt檔於D槽後，請務必關閉口病網頁，否則視同作弊) • 作答完成後，檢查無誤後，請將個人ppt檔儲存至老師提供之USB，最後請登錄USB之號碼於點名簿上，方可離開電腦教室 Ref. 4

  44. 請寫下題目1-8之病理診斷 請以英文作答，錯字扣1分 Ref. 4

  45. 請標示下列病理特徵 總得分 = A + B = Ref. 4

  46. 從OlyVIA下載圖片之方法(1) Ref. 4 點選Copy Display to Clipboard，複製適合的圖片

  47. 從OlyVIA下載圖片之方法(2) Nevus cells Back Ref. 4

  48. 從OlyVIA下載圖片之方法(3) C. 請從題目1-8中找出並標示 Nevus cells 之病理特徵 貼上所選擇之圖片後， 插上標誌，如星號 (可插上多個星號) 貼上所選擇之圖片後， 插上標誌，如箭號 Ref. 4

  49. H.請從題目1-8中找出並標示Abnormal mitosis (009)之病理特徵(請自我練習) Ref. 4 <注意>請勿複製新投影片

  50. 作答完成 • 請務必更改ppt檔名為自己的姓名與學號，如 ’陳大明9512345’ • 再將此ppt檔儲存至老師提供之USB • 最後請登錄USB之號碼於點名簿上，方可離開電腦教室 Ref. 4

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