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  1. Real-time quantitative PCR mRNA expression of vascular endothelial growth factor (VEGF) was determined using the ABI Prism 7700 sequence detection system (Applied Biosystems, Foster City, CA, USA). The change in the expression was calculated by standardizing RNA levels corrected for glyceraldehyde-3-phosphate dehydrogenase expression in the sample. Primers and probes for VEGF (NM_031836) were as follows: rat VEGF forward primer, 5-AGAGATGAGCTTCCTGCAGCATA-3; reverse primer, 5-ACATTTACACGTCTGCGGATCTT-3; TaqMan probe, 5-FAM-CCGCTCTGAACAAGGCTCACAGTGAAC-TAMRA-3. * 400 † 300 † ‡ VEGF mRNA expression (% control) 200 100 0 Cont Nif (-) Azl AII Supplemental figure. Effects of azelnidipine or nifedipine on chronic AII-induced VEGF mRNA expression. VEGF mRNA expression was measured by quantitative real-time PCR. n=6 in each group. *P < 0.05 vs. control rats, †P < 0.05 vs. AII-infused rats, ‡P < 0.05 vs. nifedipine treated AII-infused rats.

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