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DNA Biotechnology: Cloning & Genetic Recombination in AP Biology

Learn about the process of cloning and genetic recombination using restriction enzymes, creating "sticky ends" on DNA molecules, combining DNA sources, reintroducing the recombined plasmid, and growing bacteria. Discover the use of polymerase chain reaction, DNA gel electrophoresis, RFLPs and banding, the Human Genome Project, gene therapy, new DNA-based medicines, crime scene investigations, environmental clean-up, and agriculture livestock.

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DNA Biotechnology: Cloning & Genetic Recombination in AP Biology

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Presentation Transcript

  1. Pre – AP Biology DNA Biotechnology (4.4) Part 1

  2. Cloning & Genetic recombination

  3. Cutting the DNA sources using Restriction Enzymes

  4. Using Restriction Enzymes to create “stick ends” on DNA molecules

  5. Combining DNA from both sources“Sticky ends” match

  6. Reintroduce the recombined Plasmid

  7. Let the Bacteria grow and reproduce

  8. The whole process of cloning

  9. Grow bacteria for use

  10. Kary Mullis

  11. Polymerase Chain Reaction

  12. Pre – AP Biology DNA Biotechnology (4.4) Part 2

  13. DNA Gel Electrophoresis

  14. RFLPs and banding

  15. Human Genome Project

  16. Gene Therapy to treat diseases

  17. New DNA based Medicines

  18. Crime Scene Investigations

  19. Environmental Clean-up in the gulf

  20. Agriculture

  21. Livestock

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