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WP3: Evaluation Co-ordinator-P2: GEVES (Cadot Valérie)

WP3: Evaluation Co-ordinator-P2: GEVES (Cadot Valérie) Variety and seed, study and control group Groupe d’Etude et de contrôle des Variétés Et des Semences. Objectives. To evaluate germplasm for pests and diseases resistance and for quality and abiotic characters

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WP3: Evaluation Co-ordinator-P2: GEVES (Cadot Valérie)

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  1. WP3: Evaluation Co-ordinator-P2: GEVES (Cadot Valérie) Variety and seed, study and control groupGroupe d’Etude et de contrôle des Variétés Et des Semences

  2. Objectives • To evaluate germplasm for pests and diseases resistance and for quality and abiotic characters • To include the evaluations in the databases

  3. Milestones for WP3

  4. Deliverables for WP3

  5. Lettuce : 115 accessions • Disease resistance: 57 accessions • Bremia lactucae : P4 (CZ): 30 with 20 isolates (seedling tests) P10 (GBR): 17 (field resistance) • Root-knot nematodes (Meloidogyne sp.): P13 (SLO): 10 • Quality and abiotic characters: >58? • Post harvest discoloration P1(GBR): 24 • Winter hardiness P7 (AUT): 15 • Taste and other quality characters P9 (SLO) : 10 (WP3?) / (P7 (AUT): 15 in WP4 ?)

  6. Spinach: 320 acessions • Disease resistance: 267 instead of 250 • Downy mildew (Peronospora farinosa): 3-5 physio: P0 (NLD): 267 • Quality and abiotic characters: 70 • Nitrate, oxalic acid, dry matter :P5 (DAN, SW)

  7. Chicory: 265 accessions • Disease resistance:165 • Thielaviopsis basicolaP2 (FRA): 75 • Alternaria cichorii P2 (FRA): 60 • Sclerotinia sclerotiorumP2 (FRA): 30 • Quality and abiotic characters: 100? • Shape, color, taste, history, regionalism: P8 (CHE):100 (WP3?) • Sesquiterpenes: P12 (ITA): 100 • External and internal sensory characteristics P12:25 (WP3?)

  8. Minor crops: 49 accessions • Valerianella: 9 accessions • Winter hardiness, taste: P9: 10 (WP3?) • Rucola: 40 accessions • Nitrate, dry matter P5 (DAN, SW): 20 • Glucosinolate content P12 (ITA): 20 to 40

  9. Evaluation for P0 (CGN) • What has been done: Start made of discussing the evaluation of spinach accessions with the breeding companies involved for the resistance to Peronospora farinosa . • What will be done in 2008: • Finalize the plans for the evaluation of spinach accessions with the breeding companies involved. • Evaluate 267 spinach accessions for 2 Peronospora physio’s. Other physio’s will follow in 2009 and 2010

  10. Evaluation for P1 (WHRI) What has been done: • Lettuce: assessment of 24 accessions for post harvest discolouration (browning/pinking) a year early.Significant differences were found between the accessions for mean discolouration. • What will be done: Complete the analysis of the data in 2008

  11. Evaluation for P2 (GEVES) • What has been done: • chicory accessions selected for the year 2008: • For P2: 25 accessions for Thielaviopsis basicola and for Alternaria cichorii , 15 forSclerotinia sclerotiorum • For P12: 25 accessions including italian populations for sesquiterpenes lactones and internal sensory characteristics, • For P8: 25 accessions for the shape, color, taste, history regionality. • What will be done in 2008: • Start evaluations • Enter the data in a fixed format

  12. Evaluation for P4 (Palacky) • What has been done: Selection of 30 lettuce accessions for characterization, • In June: Control of viability and pathogenicity of races of Bremis lactucae, pre-selection of isolates to be used for evaluation in a year 2. • What will be done: Evaluation starts in year 2.

  13. Evaluation for P5 (NGB) • What has been done: • Spinach accessions selected for the evaluation 2008 about Nitrate, oxalic acid and dry matter • No information about the 20 accessions of Rucola about nitrate and dry matter? • What will be done: • Finalized the plans for evaluation of spinach and minor crops • Evaluations will start in 2008.

  14. Evaluation for P7 (Arche Noah) • What has been done: First selection of 15 accessions from Arche Noah collections for winter hardiness. • What will be done: • Evaluation of 15 lettuce accessions for winter hardiness in 2008 • 2 locations and 3 variations of field trials(2 outside and 1 in plastic house)

  15. Evaluation for P8 (ProSpecieRara) • What has been done: • Reception of 25 chicory accessions from P2 • ? • What will be done: • Chicory: evaluation of shape color taste history and regionallity: 25 in year 2 over 100?

  16. Evaluation for P9 (USOFA) • What has been done: • About taste, colour, appearance, shape (10 accessions?): evaluation for important property and quality for us? • What will be done:

  17. Evaluation for P10 (HDRA) • What has been done: • 17 accessions of lettuce selected (Heritage Seed Library accessions) for the evaluation to field resistance to Bremia: . • What will be done: • 2008: evaluation of the 17 accessions of lettuce to assess field Bremia resistance.

  18. Evaluation for P12 (University of Bologna) • What has been done: • Approval of the list of 25 accesions of Chicory proposed by P2 • What will be done: • Chicory: Evaluation of 25 accessions in year 2 for sesquiterpene lactones, and for external and internal sensory characteristics (seeds to send by GEVES and IPK) • Rucola: Evaluation for glucosinolate (seeds to send by IPK); Number? 20 to 40 ? • Bottlenecks: • co-workers are not permanent employees, and are seeking for more stable jobs.

  19. Evaluation for P13 (KIS) • What has been done: • Methodology for the evaluation of lettuce resistance to root knot nematodes was proposed. • Multiplication of nematodes on artificial medium, screening for purity of nematode populations, preparation of inoculum. • 10 morphologically diverse lettuce accessions from the KIS genebank collection were selected for the evaluation of resistance to root knot nematodes. • What will be done: • sowing of selected lettuce accessions for evaluation of resistance.

  20. Inquiry about the scale of notation for WP3 *Which scale of notation ? * Which data format will be sent? • A note with a level of expression associated. Ex : 1= susceptible ; 9 = resistant • A score. Ex : Necrotic Root Index, % infection, classes of necrosis,… • A file linked to the accession number but not advised to sort out • A text included in the database as “Remarks” • A combination of these 4 possibilities: Indicate which combination you wish. Ex : 5=1+2+4

  21. Scale for pests and diseases : scores and remarks

  22. Scale for quality and abiotic characters :Files or scores [XXX1]For harmonization, it would be better to define 0=absent and 1 = present) [XXX1]For harmonization, it would be better to define 0=absent and 1 = present) [XXX1]For harmonization, it would be better to define 0=absent and 1 = present)

  23. Scale for quality and abiotic characters : a few answers 2/2

  24. Proposal about the scale of notation • For disease resistance : • A binary or ternary form corresponding to the interpretation of the level of resistance: • S: Susceptible • R: Resistant • (IR : Intermediary resistance) Included in Excel as another character to have the possibility to search on this character • a file linked to the accession comprising : • The quantitative score • A field Remarks (Table, text : ex Resistance by HR…) • For quality and abiotic characters • A file linked to the accession

  25. Conclusion for WP3 • No apparent critical delay • Confusion about deliver WP3 and WP4 • What will be done in year 2: • Start or go on evaluations • Validate the fixed format, • Computerize the data and send it to the DB manager

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