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Towards new enzymes: From Triosephosphate Isomerase to Kealases

Towards new enzymes: From Triosephosphate Isomerase to Kealases. Peter Neubauer University of Oulu 31.08.2006 (PDB2006). Introduction. Enzyme of the glycolysis pathway W ild type enzyme is a dimer that consists of two identical (  )8-fold subunits with the size of 250 residues

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Towards new enzymes: From Triosephosphate Isomerase to Kealases

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  1. Towards new enzymes: From Triosephosphate Isomerase to Kealases Peter NeubauerUniversity of Oulu31.08.2006 (PDB2006)

  2. Introduction • Enzyme of the glycolysis pathway • Wild type enzyme is a dimer that consists of two identical ()8-foldsubunitswith the size of 250 residues • Loop-1 and 4 (subunit 1) and loop-3 (subunit 2) form the dimer interfase • Highly specific binding pocket (phosphate) is formed by loops 6, 7 and 8 • Catalyses the inter-conversion of dihydroxy acetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (DGAP) Peter Neubauer Norledge et al; Proteins: structure, function and genetics (2001)

  3. Loop6 Loop6 Loop7 Loop7 Catalytic residues: Lysine 13 Histidine 95 Glutamate 167 Ligand is represented as green density Loop-6 closes upon ligand binding Reaction mechanism as proposed by Kursula et al. Eur. J. Biochem (2001) Introduction Catalytic residues: Lysine 13 Histidine 95 Glutamate 167 Ligand is represented as green density Loop-6 closes upon ligand binding Peter Neubauer

  4. e.g. Wierenga (2001) Borchert et al (1993; 1994) Thanki et al. (1997) Dimer to monomer • Dimeric stabilization is important for the catalytic machinery in wild type TIM • MonoTIM and ml1 TIM are active enzymes and catalyze the substrates in a similar fashion • The affinity of the monomeric TIMs for the transition state analogues is lower • The turnover number for the monomeric TIMs is lower • Ml8b TIM does no longer convert any known substrates. However the active site remains the same. ml8b TIMNorledge etl. (2001) Peter Neubauer

  5. Monomeric TIM activity • The turnover number is 1000 lower in monomeric TIM compared to wild type This is correlated with a different environment, such as an increased solvent accessibility • The reduction in affinity for 2PG is less than for the phosphate moiety • Lysine 13 and Histidine 95 show more conformational flexibility compared to wild type. Monomeric TIMs are more “floppy“ • The more accessible active site is an interesting starting point for enzyme engineering Loop-6 Glu 167 2PG His 95 Lys 13 Mode of binding of 2PG in wild type and monomeric TIM (ml1 TIM). Peter Neubauer

  6. Tools for new enzymes Importance of chirally active aldehydes • What? • Design of new artificial enzymes • Design and organic synthesis of new substrates • Creating active enzymes Mass Spectrometry Wild type studies X-Raycrystallography Chemistry NMR Multidisciplinary approach of the biocatalyst consortium Pool of enzymes Screen for activity Our Start: Our goal: TIM (variants)[inactive] Input Output Kealases (Random) mutagenesis Selection of best mutants • iterative process Wild type studies Peter Neubauer

  7. Recent wild type studies • Investigations on the properties of the conserved active site proline identified a molecular switch to bring the enzyme in a competent state for catalysis upon ligand binding. • Enzymology studies on P168A mutant versus wild type show a dramatic drop in Kcat/Km • The affinity for 2PG is reduced • The mode of binding of 2PG in wild type differs from P168A mutant. • Conclusions: • Proline 168 is needed for conformational strain around the catalytic glutamate. This strain is transferred to the catalytic glutamate. • Ligand binding triggers the conformational switch of the catalytic machinery needed for catalysis. Poster:”The functional role of the conserved active site proline of triosephosphate isomerase” Peter Neubauer

  8. Why monomeric TIM? Monomeric TIM is a very suitable protein for biocatalysis: • small size (suitable for NMR; easy to crystallize): so far all constructs of monomeric TIMs could be crystallised) • easily expressed in high amounts of soluble protein in E.coli • Monomeric protein has advantages (Vanvaca et al. PNAS 2004) • No cofactors needed • In the closed conformation the binding site is an extended groove: ml8b TIM (*) • Its wild type precursor is the extensively studied TIM. L Peter Neubauer

  9. (cleavable) Anchor Catalytic head group Catalytic Extended binding pocket Original binding site - S i t e Tailored ligands The Concept • Catalytic head group stays the same • Achor part contributes most to the binding affinity • The anchor is optimized for maximum affinity, but can be cleavable Enzyme surface A possible Substrate A Possible Inhibitor Peter Neubauer

  10. Active site Extended groove A-TIM • Over 20 constructs based on ml8b TIM have been created • Several mutations have been investigated: pocket, rim, active site and loop mutants. Many have structures have been solved • A variant with a deeper and wider binding groove have been created: A-TIM • A-TIM is as stable as ml1 TIM in solution • The active site is identical to wild type TIM Peter Neubauer

  11. Saab-Rincon et al. Protein eng. (2001): Poster:Non-natural enzymes: Directed evolution of a monomeric triosephosphate isomerase variant – a case study A-TIM • Over 35 novel tailored ligands have been created • Binding studies with NMR, Surface Plasma Resonance and X-ray crystallography have identified lead compounds which can be called “binders” • Site directed evolution is the next logical step to convert “binders“ to substrates • Site directed evolution has worked previously to improve activity • If A-TIM variants converts α-hydroxy ketones to α-hydroxy aldehydes, they can be called Kealases Peter Neubauer

  12. 1.06 Å detail of A-TIM variant Recent findings • In wild type Proline 168 acts as a molecular switch upon ligand binding. Manuscript is submitted • A study on C-terminal hinge residue A178 in wild type and monomeric TIM provides interesting details about the “floppy“ nature of monomeric TIM. Manuscript is under preparation • An atomic resolution structure of wild type TIM with PGH (0.82 Å) will give new insights in the reaction mechanism (data under investigation) Peter Neubauer

  13. Citric acid Malic acid MW-1 Mare, de La et al. Biochem J (1972) Recent findings • In X-ray structures the three following compounds are seen as “binders“ • In an X-Ray structure BHAP (bromo hydroxyactone phosphate; a suicide inhibitor) has been found to react in the active site similar to wild type.This finding indicates that A-TIM can still convert the original substrate (Kcat is too low to measure): • A-TIM is an active molecule Peter Neubauer

  14. A possible application for A-TIM • New A-TIM enzymes towards the transformation of new modified nucleosides • New result: citric acid binds to the active site of A-TIM (structure 1.5 Å) • Also malic acid and compound MV-1 bind to the active site (seen in X-ray) • Surface plasma resonance method to screen for binders has been established (Dr. Päivi Pirila) • Citric acid as positive control binds in the assay specifically • Other compounds tested (with good results): Citric acid Malic acid MW-1 Peter Neubauer

  15. Acknowledgments Faculty of Science Department of Biochemistry Prof. Rik WierengaMarkus AlahuhtaMikko SalinVille Ratas Department of Chemistry Prof. Jouni PursiainenRitva JuvaniProf. Marja LajunenMatti VaismaaDr. Sampo MattilaNanna Alho Faculty of Technology Bioprocess Engineering Prof. Peter NeubauerDr. Mari YlianttilaMarco CasteleijnLilja Kosamo Collaborators University of Kuopio Department of Pharmaceutical Chemistry Prof. Seppo Lapinjoki Technical University of Helsinki Dr. Petri Pihko University of Antwerp, Belgium Prof. Koen AugustynsProf. Anne-Marie Lambeir This work has been supported by the Academy of Finland (project 53923) and Tekes (project Biocatnuc) website:http://www.oulu.fi/bioprocess/ Peter Neubauer

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