NAME / Lamiaa Fathy Asal Specialization / poultry Advisor / prof .Dr Hassan saber zeweil

2. NAME / LamiaaFathyAsal • Specialization / poultry • Advisor / prof .Dr Hassan saber zeweil

3. Effect of feeding diets containing probiotic

4. What are Probiotics ? The term probiotics comes from the Greek meaning "for life".  Probiotics are the billions of 'live friendly' bacteria (the good guys) that work within the digestive system of animals & humans to restore intestinal balance & maintain normal digestive health.  Microflora Millions of bacteria are lost from the digestive tract every day and because the gut always contains both 'good & bad' bacteria, it's the balance that is key. The community of good/bad bacteria are commonly called 'microflora'. 

5. Why use Probiotics? Probiotics keep the good bacteria healthy & flourishing so that digestion works at optimal levels which in turn supports the immune system.  The 'friendly bacteria' stop the potentially harmful (pathogenic) bacteria from becoming dominant, thus helping to keep the correct balance of good and bad bacteria.  In turn this can allow better use of the nutrients obtained from the daily diet and a stronger immune system.  Animals & humans alike require help breaking down food into digestible substances – the billions of friendly bacteria do this within our guts.  There is also much anecdotal evidence (including from our clients!) that they act as a highly effective natural 'calmer'.  

6. Mode of action • Competing against pathogenic bacteria for nutrients in the gut • Competing with pathogens for binding sites on the intestinal wall • Producing compounds that are toxic to pathogens • Stimulating the immune system so it is ready to fight invading pathogens

7. Assessment of the effects of supplementing rabbit diets with a culture of Saccharomycescerevisiae using growth performance, blood composition and clinical enzyme activities A.A. Onifade1,*, R.I. Obiyan, E. Onipede, D.O. Adejumo, O.A. Abu, G.M. Babatunde Department of Animal Science, University of Ibadan, Ibadan, Nigeria

8. EXPERIMENTAL Diets • 3.0 gkg-1 yeast • 10 rabbits • 0.0 gkg-1 yeast • 10 rabbits • 1.5 gkg-1yeast • 10 rabbits

9. Table 1 Composition of the basal diet fed to weanling rabbits aYeasacc10261 containing 108 cfu g-1 of Saccharomyces cerevisiae was added to separate batches of the basal diet at 0.0, 1.50 and 3.0 g kg-1 to provide dietary treatments 1, 2 and 3, respectively. bProvided per kilogram of diet: vitamin A, 10 000 IU (retinyl acetate); cholecalciferol, 3000 IU; vitamin E, 8.0 IU (DL-a-tocopheryl acetate); K, 2.0 mg; thiamine, 2.0 mg; pyridoxine, 1.2 mg; cyanocobalamin, 0.12 mg; niacin, 1.0 mg; pantothenic acid, 7.0 mg; folic acid, 0.6 mg; choline chloride, 500 mg; Fe, 60 mg; Mn, 100 mg; Cu, 8.0 mg; Zn, 50 mg; Co, 0.45 mg; I, 2.0 mg; Se, 0.1 mg.

10. Results

11. Table 2 Performance characteristics of rabbits fed basal or yeast-supplemented diets a-cMeans s in the same row without similar supercripts are significantly (P <0.05) different. *: P < 0.05; **: P < 0.01; ***: P < 0.001 NS: Not significant

12. Table 3 Blood composition in rabbits fed basal or yeast-supplemented diets 1MCV, mean corpuscular volume; MCH, mean corpuscular haemoglobin; MCHC, mean corpuscular haemoglobin concentration. 2Leucocyte differentials are expressed as percentage of the total cells count. a±c: Means in the same row without similar supercripts are significantly (P<0.05) different. *: P < 0.05; **: P < 0.01; ***: P < 0.001. NS: Not significant

13. Table 4 Serum clinical chemistry of rabbits fed basal or yeast-supplemented diets 1AST, Aspartate aminotransferase; ALT, Alanine aminotranferase; ALP, Alkaline phosphatase. a-cMeans in the same row without similar superscripts are significantly (P < 0.05) different. *: P < 0.05; **: P < 0.01; ***: P < 0.001. NS: Not significant

14. Conclusion

15. It can be concluded from this study that the addition of a culture of Saccharomycescerevisiae had a growth stimulating effect and the responses were generally linearly related to the concentration of yeast.

16. EFFECT OF INCLUSION OF PROBIOTICS ON MICRO-RGANISMS CONTENT, HEALTH AND PERFORMANCE OF FATTENING RABBITS: 1. STUDY IN A COMMERCIAL FARM WITH INTERMEDIATE HEALTH STATUS Kritas S.K.1*, Petridou E.1, Fortomaris P.2, Tzika E.3, Arsenos G.2, Koptopoulos G.1 1Department Microbiology & Infectious Diseases, Veterinary School, Aristotle University of Thessaloniki, GR-54121, Thessaloniki, Macedonia, Greece 2Department Animal Husbandry, Veterinary School, Aristotle University of Thessaloniki, GR-54121, Thessaloniki, Macedonia, Greece 3Clinic of Productive Animal Medicine, Veterinary School, Aristotle University of Thessaloniki, GR-54121, Thessaloniki, Macedonia, Greece *Corresponding author: skritas@vet.auth.gr

17. EXPERIMENTAL Diets EXPERIMENTAL Diets • Control group • 840 rabbits • Probiotic group • 840 rabbits

18. Results

19. Table 1: Presence of Escherichia coli(>107 cfu/g), Clostridium perfringens (>107 cfu/g) and Pasteurella multocida in the faeces, intestine and lungs of fattening rabbits, respectively, after administration of probiotics * Pearson chi square (P=0.05)

20. Table 2: Growth performance in the groups of rabbits at different fattening stages * Different superscripts in the same row denote statistically significant difference (P≤0.05)

21. Conclusion

22. The results of this study showed that administration of the probioticBioPlus 2B at 400 g/T of feed to fattening rabbits from 4 days post weaning until 5 days before the slaughter age reduces mortality and the presence of E. coli and C. perfringens in the faeces, and improves growth performance (ADG and FCR)..