1 / 9

Isolation and Enrichment of soil bacteria

Isolation and Enrichment of soil bacteria. Regina Gohl, K. Noreen Boyle 02/11/2011. University of Oldenburg - ICBM - Practical course Physiology of bacteria (Group B1) – final presentation. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 2. Introduction.

Download Presentation

Isolation and Enrichment of soil bacteria

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Isolation andEnrichmentofsoilbacteria Regina Gohl, K. Noreen Boyle 02/11/2011 University of Oldenburg - ICBM - PracticalcoursePhysiologyofbacteria (Group B1) – final presentation

  2. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 2 Introduction • 1 soil probe  2 experiments Isolation Enrichment ? 37 °C Pasteurisation Nitrogen Nitrogen + yeast Salt Salt+yeast Acidic Alkaline Dilution series Medium variation Introduction Aims & Expectations Courseresults Summary

  3. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 3 Aimsandexpectations • Cultivationofsoilbacteria • Determination ofabundance • Comparison: fast vs abundant • Influenceof different media Introduction Aims& Expectations Courseresults Summary

  4. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 4 Direct Isolation ~ 10^9 bacteria/g soil 2.8 x 10^6 cells/g soil ~1 % ofbacteriacan becultured in the lab Lessbacteriathanexpected Introduction Aims & Expectations Courseresults Summary

  5. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 5 Direct Isolation Introduction Aims & Expectations Courseresults Summary

  6. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 6 Enrichment http://www.laborwissen.de/wiki/ tiki-index.php?page=Katalase-Test Introduction Aims & Expectations Courseresults Summary

  7. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 7 Enrichment Introduction Aims & Expectations Courseresults Summary

  8. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 8 Summary • Resultsasexpected • Varietyofbacteria in soil • Different treatment growth / nogrowth -nitrogensource+yeastextract -nitrogensource Introduction Aims & Expectations Courseresults Summary

  9. Thankyouforyourattention

More Related